Abstract:
BACKGROUND : The use of molecular diagnostic techniques for the evaluation of the impact of pneumococcal
conjugate vaccines (PCVs) has not been documented. We aimed to evaluate the impact of PCVs on invasive
pneumococcal disease (IPD) using polymerase chain reaction (PCR)-based techniques and compare with results
obtained from culture-based methods.
METHODS : We implemented two independent surveillance programs for IPD among individuals hospitalized at one
large surveillance site in Soweto, South Africa during 2009–2012: (i) PCR-based (targeting the lytA gene) syndromic
pneumonia surveillance; and (ii) culture-based laboratory surveillance. Positive samples were serotyped. The
molecular serotyping assay included targets for 42 serotypes including all serotypes/serogroups included in the
7-valent (PCV-7) and 13-valent (PCV-13) PCV. The Quellung reaction was used for serotyping of culture-positive
cases. We calculated the change in rates of IPD (lytA- or culture-positive) among HIV-uninfected children aged
<2 years from the year of PCV-7 introduction (2009) to the post-vaccine years (2011 or 2012).
RESULTS : During the study period there were 607 lytA-positive and 1,197 culture-positive cases that were serotyped.
Samples with lytA cycle threshold (Ct)-values ≥35 (30.2 %; 123/407) were significantly less likely to have a serotype/
serogroup detected for serotypes included in the molecular serotyping assay than those with Ct-values <35 (78.0 %;
156/200) (p < 0.001). From 2009 to 2012 rates of PCV-7 serotypes/serogroups decreased −63.8 % (95 % CI: −79.3 %
to −39.1 %) among lytA-positive cases and −91.7 % (95 % CI: −98.8 % to −73.6 %) among culture-positive cases.
Rates of lytA-positive non-vaccine serotypes/serogroups also significantly decreased (−71.7 %; 95 % CI: −81.1 % to
−58.5 %) over the same period. Such decline was not observed among the culture-positive non-vaccine serotypes
(1.2 %; 95 % CI: −96.7 % to 58.4 %).
CONCLUSIONS : Significant downward trends in IPD PCV-7 serotype-associated rates were observed among patients
tested by PCR or culture methods; however trends of non-vaccine serotypes/serogroups differed between the two
groups. Misclassifications of serotypes/serogroups, affecting the use of non-vaccine serotypes as a control group,
may have occurred due to the low performance of the serotyping assay among lytA-positive cases with high Ctvalues.
Until PCR methods improve further, culture methods should continue to be used to monitor the effects of
PCV vaccination programs on IPD incidence.
