Studies on Dictyocaulus filaria. I. Modifications of laboratory procedures

Anderson, P.J.S.; Verster, Anna

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dc.contributor.author	Anderson, P.J.S.
dc.contributor.author	Verster, Anna
dc.contributor.editor	South Africa. Dept. of Agricultural Technical Services
dc.contributor.editor	De Lange, M.
dc.contributor.editor	Reinecke, R.K.
dc.contributor.editor	Walker, Jane B.
dc.contributor.editor	De Kock, V.E.
dc.contributor.editor	Howell, P.G.
dc.date.accessioned	2015-11-30T07:46:44Z
dc.date.available	2015-11-30T07:46:44Z
dc.date.created	2015
dc.date.issued	1971
dc.description	The journals have been scanned in colour with a HP 5590 scanner; 600 dpi. Adobe Acrobat v.11 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.	en_ZA
dc.description.abstract	Techniques used in a study of the life-cycle of Dictyocaulus filaria (Rudolphi, 1809) are described. First stage larvae migrate from faecal pellets placed in water. Infective larvae are cultured in clean water at room temperature and aerated overnight for 7 days. Sheep are infested per os with infective larvae. At various intervals after infestation they are killed and their organs are cut into small pieces, placed in a modified Baermann apparatus and the parasites allowed to migrate from the tissue into physiological saline at 37°C for 6 to 8 hours. Larvae are collected from lymph drained from the thoracic duct with a catheter. Some of the recovered worms are killed with an iodine solution; others intended for morphological studies are killed by heating to 60°C and preserved in a mixture containing triethanolamine.	en_ZA
dc.identifier.citation	Anderson, PJS & Verster, A 1971, 'Studies on Dictyocaulus filaria. I. Modifications of laboratory procedures’, Onderstepoort Journal of Veterinary Research, vol. 38, no. 3, pp. 181-4.	en_ZA
dc.identifier.uri	http://hdl.handle.net/2263/50989
dc.language.iso	en	en_ZA
dc.publisher	Pretoria : Government Printer	en_ZA
dc.rights	©South Africa. Dept. of Agricultural Technical Services (original). ©University of Pretoria. Dept. of Library Services (digital).	en_ZA
dc.subject	Veterinary medicine	en_ZA
dc.subject	South Africa	en_ZA
dc.subject.lcsh	Veterinary medicine -- South Africa
dc.title	Studies on Dictyocaulus filaria. I. Modifications of laboratory procedures	en_ZA
dc.type	Article	en_ZA