Plaque formation by African horsesickness virus and characterization of its RNA

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dc.contributor.editor South Africa. Dept. of Agricultural Technical Services
dc.contributor.editor Tustin, R.C.
dc.contributor.editor De Lange, M.
dc.contributor.editor Reinecke, R.K.
dc.contributor.editor Walker, Jane B.
dc.contributor.editor De Kock, V.E.
dc.contributor.upauthor Oellermann, R.A.
dc.date.accessioned 2015-07-08T11:23:17Z
dc.date.available 2015-07-08T11:23:17Z
dc.date.created 2013
dc.date.issued 1970
dc.description The journals have been scanned in colour with a HP 5590 scanner; 600 dpi. Adobe Acrobat v.11 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. en
dc.description.abstract A method of plaque assay for African horsesickness virus (AHSV) is described. Three cell lines were tested of which BHK21 was the most satisfactory. With an increase in Actinomycin D concentration, an increased inhibition of infective virus production was observed. Sucrose gradient sedimentation analysis of AHSV-RNA revealed at least six components. Eight components were resolved by polyacrylamide gel electrophoresis. The molecular weight of the components varied from 0.5 x 10⁶ to 2.8 x 10⁶ daltons, with a total molecular weight estimate of 1.5 x 10⁷ daltons for 10 segments in the viral genome. The significance of the relationship between AHSV, bluetongue virus and reovirus is discussed. en
dc.description.librarian mn2015
dc.description.sponsorship . en
dc.identifier.citation Oellermann, RA 1970, 'Plaque formation by African horsesickness virus and characterization of its RNA', Onderstepoort Journal of Veterinary Research, vol. 37, no.2 , pp. 137-144. en
dc.identifier.uri http://hdl.handle.net/2263/46332
dc.language.iso en en
dc.publisher Pretoria : Government Printer en
dc.rights ©South Africa. Dept. of Agricultural Technical Services (original). ©University of Pretoria. Dept. of Library Services (digital). en
dc.subject Veterinary medicine en
dc.subject Veterinary reports en
dc.subject South Africa en
dc.subject.lcsh Veterinary medicine -- South Africa en
dc.subject.lcsh African horse sickness en
dc.subject.lcsh Horses -- Virus diseases en
dc.subject.lcsh Orbivirus infections in animals en
dc.title Plaque formation by African horsesickness virus and characterization of its RNA en
dc.type Article en


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