dc.contributor.editor |
South Africa. Dept. of Agricultural Technical Services |
|
dc.contributor.editor |
Tustin, R.C. |
|
dc.contributor.editor |
De Lange, M. |
|
dc.contributor.editor |
Reinecke, R.K. |
|
dc.contributor.editor |
Walker, Jane B. |
|
dc.contributor.editor |
De Kock, V.E. |
|
dc.contributor.upauthor |
Oellermann, R.A.
|
|
dc.date.accessioned |
2015-07-08T11:23:17Z |
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dc.date.available |
2015-07-08T11:23:17Z |
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dc.date.created |
2013 |
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dc.date.issued |
1970 |
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dc.description |
The journals have been scanned in colour with a HP 5590 scanner; 600 dpi. Adobe Acrobat v.11 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. |
en |
dc.description.abstract |
A method of plaque assay for African horsesickness virus (AHSV) is described. Three cell lines were tested of which BHK21 was the most satisfactory. With an increase in Actinomycin D concentration, an increased inhibition of infective virus production was observed. Sucrose gradient sedimentation analysis of AHSV-RNA revealed at least six components. Eight components were resolved by polyacrylamide gel electrophoresis. The molecular weight of the components varied from 0.5 x 10⁶ to 2.8 x 10⁶ daltons, with a total molecular weight estimate of 1.5 x 10⁷ daltons for 10 segments in the viral genome. The significance of the relationship between AHSV, bluetongue virus and reovirus is discussed. |
en |
dc.description.librarian |
mn2015 |
|
dc.description.sponsorship |
. |
en |
dc.identifier.citation |
Oellermann, RA 1970, 'Plaque formation by African horsesickness virus and characterization of its RNA', Onderstepoort Journal of Veterinary Research, vol. 37, no.2 , pp. 137-144. |
en |
dc.identifier.uri |
http://hdl.handle.net/2263/46332 |
|
dc.language.iso |
en |
en |
dc.publisher |
Pretoria : Government Printer |
en |
dc.rights |
©South Africa. Dept. of Agricultural Technical Services (original). ©University of Pretoria. Dept. of Library Services (digital). |
en |
dc.subject |
Veterinary medicine |
en |
dc.subject |
Veterinary reports |
en |
dc.subject |
South Africa |
en |
dc.subject.lcsh |
Veterinary medicine -- South Africa |
en |
dc.subject.lcsh |
African horse sickness |
en |
dc.subject.lcsh |
Horses -- Virus diseases |
en |
dc.subject.lcsh |
Orbivirus infections in animals |
en |
dc.title |
Plaque formation by African horsesickness virus and characterization of its RNA |
en |
dc.type |
Article |
en |