An ELISA using an SDS extract of Brucella abortus strain 99 as antigen to detect B. abortus antibodies in cattle sera

Williamson, Catherine C.; Oberem, P.T.; Poerstamper, C.; De Waal, D.T.; Matthee, O.; Brett, O.L.

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dc.contributor.author	Williamson, Catherine C.
dc.contributor.author	Oberem, P.T.
dc.contributor.author	Poerstamper, C.
dc.contributor.author	De Waal, D.T.
dc.contributor.author	Matthee, O.
dc.contributor.author	Brett, O.L.
dc.contributor.editor	Bigalke, R.D.
dc.date.accessioned	2014-10-07T10:29:29Z
dc.date.available	2014-10-07T10:29:29Z
dc.date.created	2014
dc.date.issued	1988
dc.description	The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.	en
dc.description.abstract	An ELISA was developed using an SDS extract of Brucella abortus as antigen to detect antibodies in cattle sera. The antigen was stable at 4 °C for at least 4 years and although it gave optimal results at a 1/4 000 dilution it could detect reactors at a 1/32 000 dilution. Based on comparative CF tests on 430 sera from negative herds and 187 sera from positive herds a reading of 0,07 or less using a 492 nm filter could be considered a negative reaction. The ELISA could be a useful test to supplement the CF test but further evaluation is still required.	en
dc.description.librarian	lmchunu2014	en
dc.description.librarian	mn2014
dc.identifier.citation	Williamson, CC, Oberem, PT, Poerstamper, C, De Waal, DT, Matthee, O, & Brett, OL 1988, 'An ELISA using an SDS extract of Brucella abortus strain 99 as antigen to detect B. abortus antibodies in cattle sera', Onderstepoort Journal of Veterinary Research, vol. 55, no. 1, pp. 1-3.	en
dc.identifier.issn	0330-2465
dc.identifier.uri	http://hdl.handle.net/2263/42270
dc.language.iso	en	en
dc.publisher	Published by the Government Printer, Pretoria.	en
dc.rights	©ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©University of Pretoria. Dept. of Library Services (digital).	en
dc.subject	Veterinary medicine	en
dc.subject.lcsh	Veterinary medicine -- South Africa
dc.title	An ELISA using an SDS extract of Brucella abortus strain 99 as antigen to detect B. abortus antibodies in cattle sera	en
dc.type	Article	en