Serological differentiation of five bluetongue virus serotypes in indirect ELISA

Du Plessis, D.H.

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1992 Onderstepoort Journal of Veterinary Research, Volume 59, 1992
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dc.contributor.author	Du Plessis, D.H.
dc.contributor.editor	Verwoerd, Daniel Wynand
dc.date.accessioned	2014-08-21T06:47:01Z
dc.date.available	2014-08-21T06:47:01Z
dc.date.created	2013
dc.date.issued	1992
dc.description	The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.	en
dc.description.abstract	The serological reactivity in indirect ELISA of five different bluetongue virus (BTV) serotypes (4, 10, 15, 16 & 20) was compared using polyclonal antisera raised against virus particles and an outer structural protein, VP2. Rabbit and sheep antisera against BTV-10 produced higher ELISA values with their homologous antigens than with heterologous serotypes. A hyperimmune rabbit serum specific for virus particles was able to distinguish heterologous serotypes from each other, but a sheep serum from an infected animal was not. An antiserum directed against VP2, the protein responsible for serotype specificity in neutralization tests, was not serotype-specific in ELISA and cross-reacted with other serotypes. The discriminatory ability of a BTV-4 antiserum was improved by cross-absorption with heterologous antigens. This greatly reduced the ELISA signals with heterologous serotypes and produced an antiserum that was effectively serotype-specific.	en
dc.description.librarian	mn2014
dc.identifier.citation	Du Plessis, DH 1992, 'Serological differentiation of five bluetongue virus serotypes in indirect ELISA'. Onderstepoort Journal of Veterinary Research, vol. 59, no. 2, pp. 119-122.	en
dc.identifier.issn	0330-2465
dc.identifier.uri	http://hdl.handle.net/2263/41461
dc.language.iso	en	en
dc.publisher	Published by the Agricultural Research Council, Onderstepoort Veterinary Institute.	en
dc.rights	©South Africa. Dept. of Agricultural Technical Services. ©University of Pretoria. Dept. of Library Services (digital).	en
dc.subject	Veterinary medicine	en
dc.subject	BTV	en
dc.subject.lcsh	Veterinary medicine -- South Africa
dc.title	Serological differentiation of five bluetongue virus serotypes in indirect ELISA	en
dc.type	Article	en