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dc.contributor.upauthor | Joubert, Annie M. | |
dc.contributor.upauthor | Marais, Sumari | |
dc.date.accessioned | 2007-10-24T05:03:50Z | |
dc.date.available | 2007-10-24T05:03:50Z | |
dc.date.issued | 2007-05 | |
dc.description.abstract | The effects of 1 × 10-6 M exogenous 2-methoxyestradiol (2 ME) were determined on cell morphology and cell division cycle (Cdc) 2 kinase activity in SNO oesophageal carcinoma cells. Mitotic indices revealed an increase in metaphase cells (11.2%) when compared to the 0.5% vehicle-treated cells after 18 h of exposure to 2 ME. Vehicle-treated control cells did not show any hallmarks of apoptosis after 18 h of exposure to dimethyl sulphoxide. Only 0.5% of 2 ME-treated cells showed characteristics of apoptosis. Conversely, increased morphological hallmarks of apoptosis were observed in SNO-treated cells after 21.5 h of 2 ME exposure. When compared to the 0.5% in vehicle-treated cells, 4.7% of cells were in apoptosis. Furthermore, 34.1% of cells were blocked in metaphase after 21.5 h of 2 ME exposure compared to 0.6% of vehicle-control cells. In addition, Cdc2 kinase activity was statistically significantly increased (1.3-fold) (p < 0.005) in 2 ME-treated cells when compared to vehicle-treated controls. The present preliminary study suggests that the accumulation observed in metaphase cells and the increase in Cdc2 kinase activity caused by 2 ME are consistent with morphological hallmarks of mitotic arrest and disrupted mitotic spindle formation, thus leading to induction of apoptosis in SNO cells. | en |
dc.description.sponsorship | The Cancer Association of South Africa; Grant Number: AA614 The Medical Research Council, South Africa; Grant Number: AG374 | en |
dc.format.extent | 202491 bytes | |
dc.format.mimetype | application/pdf | |
dc.identifier.citation | Joubert, A & Marais, S 2007,'In vitro effects of 2-methoxyestradiol on cell morphology and Cdc2 Kinase activity in SNO oesophageal carcinoma cells', Cell Biochemistry and Function, vol. 25, no. 3, pp. 357-362. [http://eu.wiley.com/WileyCDA/WileyTitle/productCd-CBF.html] | en |
dc.identifier.issn | 0263-6484 | |
dc.identifier.issn | 1099-0844 | |
dc.identifier.other | 10.1002/cbf.1409 | |
dc.identifier.uri | http://hdl.handle.net/2263/3787 | |
dc.language.iso | en | en |
dc.publisher | John Wiley and Sons, Ltd. | en |
dc.rights | John Wiley & Sons, Ltd. | en |
dc.subject | 2-methoxyestradiol | en |
dc.subject | Cdc2 kinase | en |
dc.subject | Morphology | en |
dc.subject | Apoptosis | en |
dc.subject | Oesophageal carcinoma | en |
dc.subject.lcsh | Esophagus--Cancer | |
dc.subject.lcsh | Apoptosis | |
dc.subject.lcsh | Cell division | |
dc.subject.lcsh | Cells--Morphology | |
dc.subject.lcsh | Dimethyl sulfoxide | |
dc.subject.lcsh | Mitosis | |
dc.title | In vitro effects of 2-methoxyestradiol on cell morphology and Cdc2 Kinase activity in SNO oesophageal carcinoma cells | en |
dc.type | Postprint Article | en |