dc.contributor.author |
Titoy, G.A.P.
|
|
dc.contributor.editor |
Verwoerd, Daniel Wynand |
|
dc.date.accessioned |
2013-09-12T08:51:12Z |
|
dc.date.available |
2013-09-12T08:51:12Z |
|
dc.date.created |
2013 |
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dc.date.issued |
1995 |
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dc.description |
The articles have been scanned in colour with a HP Scanjet 5590; 600dpi.
Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. |
en |
dc.description.abstract |
A technique for the cryopreservation of third-stage larvae of Gaigeria pachyscelis is described. It consists of incubating sheathed infective larvae at 37°C in 40% (v/v) ethylene glycol for 7 min, followed by 2 min at 0°C in 82,8% (v/v) cryoprotectant mixture, prior to transferring the larvae to liquid nitrogen. The survival rate obtained with this technique is consistently high: 69,1% as assessed by motility. |
en |
dc.description.librarian |
mn2013 |
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dc.identifier.citation |
Titoy, GAP 1995, 'An improved technique for the cryopreservation of Gaigeria pachyscelis (Sandveld hookworm)’. Onderstepoort Journal of Veterinary Research, vol. 62, no. 3, pp. 215. |
en |
dc.identifier.issn |
0330-2465 |
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dc.identifier.uri |
http://hdl.handle.net/2263/31704 |
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dc.language.iso |
en |
en |
dc.publisher |
Published by the Agricultural Research Council, Onderstepoort Veterinary Institute |
en |
dc.rights |
© ARC-Onderstepoort (original).
© University of Pretoria. Dept of Library Services (digital). |
en |
dc.subject |
Veterinary medicine |
en |
dc.subject |
Cryopreservation |
en |
dc.subject |
Sheathed third-stage larvae |
en |
dc.subject |
Gaigeria pachyscelis |
en |
dc.subject |
Sandveld hookworm |
en |
dc.subject.lcsh |
Veterinary medicine -- South Africa |
|
dc.title |
An improved technique for the cryopreservation of Gaigeria pachyscelis (Sandveld hookworm) |
en |
dc.type |
Article |
en |