An improved technique for the cryopreservation of Gaigeria pachyscelis (Sandveld hookworm)

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dc.contributor.author Titoy, G.A.P.
dc.contributor.editor Verwoerd, Daniel Wynand
dc.date.accessioned 2013-09-12T08:51:12Z
dc.date.available 2013-09-12T08:51:12Z
dc.date.created 2013
dc.date.issued 1995
dc.description The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. en
dc.description.abstract A technique for the cryopreservation of third-stage larvae of Gaigeria pachyscelis is described. It consists of incubating sheathed infective larvae at 37°C in 40% (v/v) ethylene glycol for 7 min, followed by 2 min at 0°C in 82,8% (v/v) cryoprotectant mixture, prior to transferring the larvae to liquid nitrogen. The survival rate obtained with this technique is consistently high: 69,1% as assessed by motility. en
dc.description.librarian mn2013
dc.identifier.citation Titoy, GAP 1995, 'An improved technique for the cryopreservation of Gaigeria pachyscelis (Sandveld hookworm)’. Onderstepoort Journal of Veterinary Research, vol. 62, no. 3, pp. 215. en
dc.identifier.issn 0330-2465
dc.identifier.uri http://hdl.handle.net/2263/31704
dc.language.iso en en
dc.publisher Published by the Agricultural Research Council, Onderstepoort Veterinary Institute en
dc.rights © ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital). en
dc.subject Veterinary medicine en
dc.subject Cryopreservation en
dc.subject Sheathed third-stage larvae en
dc.subject Gaigeria pachyscelis en
dc.subject Sandveld hookworm en
dc.subject.lcsh Veterinary medicine -- South Africa
dc.title An improved technique for the cryopreservation of Gaigeria pachyscelis (Sandveld hookworm) en
dc.type Article en


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