Immunohistochemical identification of Cowdria ruminantium in formalin-fixed tissue sections from mice, sheep, cattle and goats

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dc.contributor.author Jardine, J.E.
dc.contributor.author Vogel, S.W.
dc.contributor.author Van der Lugt, Jaco J.
dc.contributor.editor Verwoerd, Daniel Wynand
dc.contributor.upauthor Van Kleef, Mirinda
dc.date.accessioned 2013-09-12T08:40:36Z
dc.date.available 2013-09-12T08:40:36Z
dc.date.created 2013
dc.date.issued 1995
dc.description The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. en
dc.description.abstract An immunohistochemical staining technique in which a monospecific serum was used against the major antigenic protein-1 (MAP-1) of Cowdria ruminantium, was evaluated for the detection of C. ruminantium in formalin-fixed tissues of experimentally infected mice and field cases of heartwater in sheep, cattle and goats. Mice were infected with the mouse-pathogenic stocks: Mara, Kwanyanga, Welgevonden, Nonile, Vosloo, Kümm, Mali and Omatjenne. In all these cases and in the naturally infected cattle, sheep and goats, Cowdria colonies were identified as clearly-defined, brown-staining rickettsial colonies within the cytoplasm of endothelial cells. No positive staining was observed in the control group. This technique was shown to be reliable for detecting infection with C. ruminantium in the formalin-fixed tissues of mice and domestic ruminants. en
dc.description.librarian mn2013
dc.description.librarian mn2013
dc.identifier.citation Jardine, JE, Vogel, SW, Van Kleef, M & Van der Lugt, JJ 1995, 'Immunohistochemical identification of Cowdria ruminantium in formalin-fixed tissue sections from mice, sheep, cattle and goats’. Onderstepoort Journal of Veterinary Research, vol. 62, no. 4, pp. 277-280. en
dc.identifier.issn 0330-2465
dc.identifier.uri http://hdl.handle.net/2263/31695
dc.language.iso en en
dc.publisher Published by the Agricultural Research Council, Onderstepoort Veterinary Institute en
dc.rights © ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital). en
dc.subject Veterinary medicine en
dc.subject Cattle en
dc.subject Cowdria ruminantium en
dc.subject Formalin-fixed tissue sections en
dc.subject Goats en
dc.subject Heartwater en
dc.subject Identification en
dc.subject Immunohistochemical en
dc.subject Mice en
dc.subject Sheep en
dc.subject Staining technique en
dc.subject.lcsh Veterinary medicine -- South Africa
dc.title Immunohistochemical identification of Cowdria ruminantium in formalin-fixed tissue sections from mice, sheep, cattle and goats en
dc.type Article en


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