dc.contributor.author |
Simbi, B.H.
|
|
dc.contributor.author |
Peter, T.F.
|
|
dc.contributor.author |
Burridge, M.J.
|
|
dc.contributor.author |
Mahan, S.M.
|
|
dc.contributor.editor |
Boomker, Jacob Diederik Frederik |
|
dc.date.accessioned |
2012-01-19T07:27:15Z |
|
dc.date.available |
2012-01-19T07:27:15Z |
|
dc.date.created |
2011 |
|
dc.date.issued |
2003 |
|
dc.description |
The articles have been scanned with a HP Scanjet 8300; 600dpi, saved in TIFF format.
Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. |
en |
dc.description.abstract |
Detection of heartwater is not always easy especially because all the serological assays so far available either have poor sensitivity or specificity. The indirect MAP-1B ELISA has been reported to be the most specific test for heartwater, although it does also detect antibodies to some closely related ehrlichial agents. This study was undertaken to compare two methods for the detection of heartwater infection caused by the ehrlichial agent Ehrlichia (Cowdria) ruminantium. Fifteen cattle on a heartwater-endemic farm infested with high numbers of Amblyomma hebraeum ticks, and hence exposure to E. ruminantium infection were monitored over an 8-week period by pCS20 PCR and an indirect MAP-1B ELISA. Infection was detected by pCS20 PCR in most animals with the highest number of positives (60%) in week 6 of the study. Similarly, exposure to E. ruminantium was detected by indirect MAP-1B ELISA in some animals, with the highest number of seropositives (27%) at weeks 2 - 6 of the study. The data demonstrated a fluctuating rickettsaemia in cattle in a heartwater-endemic area. Comparison of the two tests indicated that the pCS20 PCR assay was more reliable because it detected more infections than the indirect MAP-1B ELISA and would therefore be the method of choice for detection of E. ruminantium infection. |
en |
dc.description.librarian |
mn2012 |
en |
dc.description.sponsorship |
United States Agency for International Development (USAID). |
en |
dc.identifier.citation |
Simbi, BH, Peter, TF, Burridge, MJ & Mahan, SM 2003, 'Comparing the detection of exposure to Ehrlichia ruminantium infection on a heartwater-endemic farm by the pCS20 polymerase chain reaction assay and an indirect MAP1-B enzyme linked immunosorbent assay'. Onderstepoort Journal of Veterinary Research, vol. 70, no. 3, pp. 231-235 |
en |
dc.identifier.issn |
0030-2465 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/17836 |
|
dc.language.iso |
en |
en |
dc.publisher |
Pretoria : Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the University of Pretoria, Faculty of Veterinary Science |
en |
dc.rights |
© ARC-Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original).
© University of Pretoria. Dept of Library Services (digital). |
en |
dc.subject |
Veterinary medicine |
en |
dc.subject |
Amblyomma hebraeum |
en |
dc.subject |
Ehrlichia (Cowdria) ruminantium |
en |
dc.subject |
Heartwater |
en |
dc.subject |
pCS20 PCR |
en |
dc.subject |
Rickettsaemia |
en |
dc.subject |
Indirect MAP-1B Elisa |
en |
dc.subject.lcsh |
Veterinary medicine -- South Africa |
|
dc.subject.lcsh |
Heartwater -- South Africa |
en |
dc.subject.lcsh |
Tick-borne diseases in animals |
en |
dc.title |
Comparing the detection of exposure to Ehrlichia ruminantium infection on a heartwater-endemic farm by the pCS20 polymerase chain reaction assay and an indirect MAP1-B enzyme linked immunosorbent assay |
en |
dc.type |
Article |
en |
dc.type |
Text |
en_ZA |