In vitro infection by Ehrlichia ruminantium of baby hamster kidney (BHK), Chinese hamster ovary (CHO-K1) and Madin Darby bovine kidney (MDBK) cells

Josemans, A.I.; Zweygarth, Erich

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dc.contributor.author	Josemans, A.I.
dc.contributor.editor	Boomker, Jacob Diederik Frederik
dc.contributor.upauthor	Zweygarth, Erich
dc.date.accessioned	2011-12-02T07:01:07Z
dc.date.available	2011-12-02T07:01:07Z
dc.date.created	2011
dc.date.issued	2003
dc.description	The articles have been scanned with a HP Scanjet 8300; 600dpi, saved in TIFF format. Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.	en
dc.description.abstract	The Welgevonden stock of Ehrlichia ruminantium, aetiological agent of heartwater, was propagated in baby hamster kidney (BHK) cells, Chinese hamster ovary (CHO-K1) cells and Madin Darby bovine kidney (MDBK) cells. The cultures required supplementation of the medium with cycloheximide for reliable growth of E. ruminantium. Growth of the Welgevonden stock in BHK and CHO-K1 cells could lead to the development of suspension cultures suitable for the mass production of E. ruminantium for an inactivated elementary body vaccine.	en
dc.description.sponsorship	Agricultural Research Council of South Africa. The European Union (Cowdriosis Network) Grant no. IC18-CT95-0008 (DG12-SNRD).	en
dc.identifier.citation	Zweygarth, E & Josemans, AI 2003, 'In vitro infection by Ehrlichia ruminantium of baby hamster kidney (BHK), Chinese hamster ovary (CHO-K1) and Madin Darby bovine kidney (MDBK) cells'. Onderstepoort Journal of Veterinary Research, vol. 70, no. 2, pp. 165-168.	en
dc.identifier.issn	0030-2465
dc.identifier.uri	http://hdl.handle.net/2263/17687
dc.language.iso	en	en
dc.publisher	Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria.	en
dc.rights	© ARC-Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). © University of Pretoria. Dept of Library Services (digital).	en
dc.subject	Veterinary medicine	en
dc.subject	BHK cells	en
dc.subject	CHO-K1 cells	en
dc.subject	Cycloheximide	en
dc.subject	Ehrlichia (Cowdria) ruminantium	en
dc.subject	Heartwater	en
dc.subject	In vitro cultivation	en
dc.subject	MDBK cells	en
dc.subject.lcsh	Veterinary medicine -- South Africa	en
dc.subject.lcsh	Heartwater -- South Africa	en
dc.subject.lcsh	Tick-borne diseases in animals	en
dc.title	In vitro infection by Ehrlichia ruminantium of baby hamster kidney (BHK), Chinese hamster ovary (CHO-K1) and Madin Darby bovine kidney (MDBK) cells	en
dc.type	Article	en