PALM and STORM : unlocking live-cell super-resolution

Henriques, Ricardo; Griffiths, Caron; Rego, E. Hesper; Mhlanga, Musa M.

dc.contributor.author	Henriques, Ricardo
dc.contributor.author	Griffiths, Caron
dc.contributor.author	Rego, E. Hesper
dc.contributor.author	Mhlanga, Musa M.
dc.date.accessioned	2011-10-19T06:18:49Z
dc.date.available	2011-10-19T06:18:49Z
dc.date.issued	2011-05
dc.description.abstract	Live-cell fluorescence light microscopy has emerged as an important tool in the study of cellular biology. The development of fluorescent markers in parallel with super-resolution imaging systems has pushed light microscopy into the realm of molecular visualization at the nanometer scale. Resolutions previously only attained with electron microscopes are now within the grasp of light microscopes. However, until recently, live-cell imaging approaches have eluded super-resolution microscopy, hampering it from reaching its full potential for revealing the dynamic interactions in biology occurring at the single molecule level. Here we examine recent advances in the super-resolution imaging of living cells by reviewing recent breakthroughs in single molecule localization microscopy methods such as PALM and STORM to achieve this important goal.	en
dc.description.uri	http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-0282	en_US
dc.identifier.citation	Henriques, R, Griffiths, C, Rego, EH & Mhlanga, MM 2011, 'PALM and STORM : unlocking live-cell super-resolution', Biopolymers, vol. 95, no. 5, pp. 322-331.	en
dc.identifier.issn	0006-3525 (print)
dc.identifier.issn	1097-0282 (online)
dc.identifier.other	10.1002/bip.21598
dc.identifier.uri	http://hdl.handle.net/2263/17454
dc.language.iso	en	en_US
dc.publisher	Wiley	en_US
dc.rights	© 2011 Wiley Periodicals, Inc. This is the pre-peer reviewed version of the following article: Henriques, R, Griffiths, C, Rego, EH & Mhlanga, MM 2011, 'PALM and STORM : unlocking live-cell super-resolution', Biopolymers, vol. 95, no. 5, pp. 322-331, which has been published in final form at wileyonlinelibrary.com.	en
dc.subject	Super-resolution	en
dc.subject	Cellular biology	en
dc.subject	Single molecule	en
dc.subject.lcsh	High resolution imaging	en
dc.subject.lcsh	Microscopy	en
dc.subject.lcsh	Fluorescence microscopy	en
dc.subject.lcsh	Cytology -- Research	en
dc.title	PALM and STORM : unlocking live-cell super-resolution	en
dc.type	Preprint Article	en