• Late-onset hydroxyurea-induced melanonychia and tongue hyperpigmentation in a patient with polycythemia vera : a case report 

  Letete, Nena; Vaz, Deborah (Springer, 2024-02)

  In a rare case, a 70-year-old female with polycythemia vera developed late-onset melanonychia, a seldom-documented side effect of hydroxyurea. Typically, melanonychia emerges within months of treatment, but this case is ...
• A pilot study evaluating the ex vivo effects of varying factor VIII concentration on clot kinetics and architecture in patients with haemophilia A 

  Morris, Chanel; Potgieter, Johan C.; Bester, Janette (Sage, 2023)

  BACKGROUND : Haemophilia A (HA) is a bleeding disorder, due to a deficiency in factor VIII (FVIII). These patients are unable to produce a stable fibrin clot in the propagation phase of coagulation as they do not generate ...
• Immune and metabolic alterations in children with perinatal HIV exposure 

  Du Toit, Louise de Villiers; Prinsloo, Andrea; Steel, Helen C.; Feucht, Ute Dagmar; Louw, Roan; Rossouw, Theresa (MDPI, 2023-02)

  With the global rollout of mother-to-child prevention programs for women living with HIV, vertical transmission has been all but eliminated in many countries. However, the number of children who are exposed in utero to HIV ...
• Establishment of haemoglobin A2 reference intervals in Pretoria, South Africa : a retrospective secondary data analysis 

  Nieuwenhuizen, Cailin; Netshidzivhani, Tshiphiri; Potgieter, Johan (AOSIS, 2022-08-12)

  BACKGROUND : Haemoglobinopathies are one of the most common inherited diseases worldwide. Quantification of haemoglobin A2 is necessary for the diagnosis of the beta thalassaemia trait. In this context, it is important ...
• Dolutegravir potentiates platelet activation by a calcium-dependent, ionophore-like mechanism 

  Madzime, Morris; Theron, Annette J.; Anderson, Ronald; Tintinger, Gregory; Steel, Helen C.; Meyer, Pieter Willem Adriaan; Nel, Jan Gert; Feldman, Charles; Rossouw, Theresa M. (Taylor and Francis, 2022)

  Please read abstract in the article.
• Ex vivo platelet morphology assessment of chronic myeloid leukemia patients before and after Imatinib treatment 

  Repsold, Lisa; Pool, Roger; Karodia, Mohammed; Tintinger, Gregory Ronald; Joubert, Anna Margaretha (Wiley, 2022-06)

  Chronic myeloid leukemia (CML) is a myeloproliferative disease and the first line treatment is through the administration of Imatinib, a first generation tyrosine kinase inhibitor. Thrombocytosis and bleeding irregularities ...
• Recombinant sclerostin inhibits bone formation in vitro and in a mouse model of sclerosteosis 

  Dreyer, Timothy; Shah, Mittal; Doyle, Carl; Greenslade, Kevin; Penney, Mark; Creeke, Paul; Kotian, Apoorva; Ke, Hua Zhu; Naidoo, Vinny; Holdsworth, Gill (Elsevier, 2021-07)

  BACKGROUND : Sclerosteosis, a severe autosomal recessive sclerosing skeletal dysplasia characterised by excessive bone formation, is caused by absence of sclerostin, a negative regulator of bone formation that binds LRP5/6 ...
• Apoptotic profiling of chronic myeloid leukaemia patients' platelets ex vivo before and after treatment with Imatinib 

  Repsold, Lisa; Pool, Roger; Karodia, Mohammed; Tintinger, Gregory Ronald; Becker, Piet J.; Joubert, Anna Margaretha (Wiley, 2021-06)

  Chronic myeloid leukaemia (CML) is a malignancy of the haematopoietic stem cells. The first line of treatment for CML, especially in developing countries, remains the first-generation tyrosine kinase inhibitor, Imatinib. ...
• Submission for special issue : The role of platelet activation in the pathophysiology of HIV, tuberculosis, and pneumococcal disease. Bedaquiline suppresses ADP-mediated activation of human platelets in vitro via interference with phosphatidylinositol 3-kinase 

  Tintinger, Gregory Ronald; Theron, Annette J.; Steel, Helen C.; Cholo, Moloko C.; Nel, Jan Gert; Feldman, Charles; Anderson, Ronald (MDPI, 2021-02-26)

  Although bedaquiline has advanced the treatment of multidrug-resistant tuberculosis (TB), concerns remain about the cardiotoxic potential of this agent, albeit by unexplored mechanisms. Accordingly, we have investigated ...
• Hypercoagulability in COVID-19 

  Wessels, Pieter Frederik (South African Heart Association, 2020-11)

  COVID-19 is associated with a hypercoagulable state that may present as pulmonary thrombosis, pulmonary embolism, and venous and arterial thrombosis. Suggested pathogenesis include direct infection of the endothelial cell ...
• ADP-mediated upregulation of expression of CD62P on human platelets is critically dependent on co-activation of P2Y1 and P2Y12 receptors 

  Anderson, Ronald; Theron, Annette J.; Steel, Helen C.; Nel, Jan Gert; Tintinger, Gregory Ronald (MDPI Publishing, 2020-12)

  This study probed the di erential utilization of P2Y1 and P2Y12 receptors in mobilizing CD62P (P-selectin) from intracellular granules following activation of human platelets with adenosine 50-diphosphate (ADP, 100 mol L1) ...
• The effect of freezing and thawing of samples for anti-factor Xa testing for the determination of enoxaparin activity 

  Louw, Susan; Gounden, Reenelle; Vaughan, Jenifer; Ntabeni, Lumka; Mayne, Anthony; Mayne, Elizabeth S. (Wiley, 2021-06)

  The quality and validity of laboratory results, especially for coagulation analyses, are influenced by pre-analytical variables including the collection technique, transportation, pre-analytical processing and storage of ...
• Endocrine profiling in patients with Fanconi anemia, homozygous for a FANCG founder mutation 

  Dillon, Bronwyn; Feben, Candice; Segal, David; Du Plessis, Johannes; Reynders, David; Wainwright, Rosalind; Poole, Janet; Krause, Amanda (Wiley Open Access, 2020-08)

  BACKGROUND : Fanconi anemia (FA) is phenotypically diverse, hereditary condition associated with bone marrow failure, multiple physical abnormalities, and an increased susceptibility to the development of malignancies. ...
• Home therapy for inherited bleeding disorders in South Africa : results of a modified Delphi consensus process 

  Mahlangu, J.N.; Naidoo, Y.; Goga, Y.; Vaithlingum, M.; Joubert, J.; Sutton, C.; Potgieter, Joachim; Cruickshank, A.L.; Iorio, A. (Health and Medical Publishing Group, 2019-09)

  BACKGROUND: Optimal care of patients with inherited bleeding disorders requires that bleeding episodes are treated early, or still better prevented, through extension of patient care beyond hospital-based treatment to ...
• HIV and haematopoiesis 

  Durandt, Chrisna; Potgieter, J.C.; Mellet, Juanita; Herd, Candice Lee; Khoosal, R.; Nel, Jan Gert; Rossouw, Theresa M.; Pepper, Michael Sean (Health and Medical Publishing Group, 2019-09)

  Human immunodeficiency virus (HIV) infection not only leads to a compromised immune system, but also disrupts normal haematopoiesis, resulting in the frequent manifestation of cytopenias (anaemia, thrombocytopenia and ...
• Clofazimine, but not isoniazid or rifampicin, augments platelet activation in vitro 

  Anderson, Ronald; Theron, Annette J.; Nel, Jan Gert; Durandt, Chrisna; Cholo, Moloko C.; Feldman, Charles; Tintinger, Gregory Ronald (Frontiers Media, 2018-11-20)

  Although the inclusion of the cationic amphiphilic, anti-mycobacterial agent, clofazimine, in the chemotherapeutic regimens of patients with multidrug-resistant tuberculosis (TB) has contributed to improved outcomes, ...
• Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay 

  Senamela, Tshiphiri; Kock, Marleen M.; Becker, Piet J.; Potgieter, Joachim (AOSIS Open Journals, 2018-01-31)

  The purpose of this study was to develop a real time polymerase chain reaction (PCR) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories. Sanger sequencing and a newly developed ...
• Dilute Russel viper venom time analysis in a haematology laboratory : an audit 

  Kruger, Welma; Meyer, Pieter Willem Adriaan; Nel, Jan Gert (Wiley, 2018-08)

  INTRODUCTION : To determine whether the current set of evaluation criteria used for dilute Russel Viper Venom Time (dRVVT) investigations in the routine laboratory meet expectation and identify possible shortcomings. METHODS ...
• Retrospective data analysis of all requests for flow cytometric immunophenotyping in a tertiary hospital setting 

  Prinsloo, Andrea; Nel, Jan Gert; Pool, Roger (Society of Medical Laboratory Technologists of South Africa, 2017-06)

  Flow cytometry is a globally accepted diagnostic tool used for the rapid identification of cells based on their surface and intracellular antigens, especially for the diagnosis of haematological malignancies. The aim of ...
• Cancer diagnoses profile of bone marrow specimens from November 2012 to December 2015 in a tertiary academic setting 

  Swart, Nicola; Pool, Roger; Prinsloo, Andrea (Society of Medical Laboratory Technologists of South Africa, 2017-06)

  This study aimed to examine the number and type of cancer diagnoses made based on bone marrow aspirates (BMA) using morphology and/or histology, over a three year period. All the data collected (2012-2015) was obtained ...

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