Molecular sexing of African rhinoceros

Peppin, Lindsay; McEwing, Ross; Ogden, Rob; Hermes, Robert; Harper, Cindy Kim; Guthrie, Alan John; Carvalho, Gary R.

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dc.contributor.author	Peppin, Lindsay
dc.contributor.author	McEwing, Ross
dc.contributor.author	Ogden, Rob
dc.contributor.author	Hermes, Robert
dc.contributor.author	Harper, Cindy Kim
dc.contributor.author	Guthrie, Alan John
dc.contributor.author	Carvalho, Gary R.
dc.date.accessioned	2010-03-31T08:12:56Z
dc.date.available	2010-03-31T08:12:56Z
dc.date.issued	2009-04
dc.description.abstract	We report the development of a fast and reliable PCR-based method for sex identification of African Rhinoceros (Ceratotherium simum and Diceros bicornis) that could easily be incorporated into fluorescent short tandem repeat (STR) profiling. A single primer pair, consisting of a fluorescently labelled forward primer and an unlabelled reverse primer, is used to co-amplify homologous fragments of a zinc finger (ZF) protein intron which exhibits size polymorphism between the X and Y chromosomes. In both species, the amplified ZFX and ZFY amplicons differ in size by 7 bp and can thus be differentiated by capillary electrophoresis. Blood, tissue, horn, and faecal samples were correctly sexed using this method. Cross species testing also demonstrated that this method could be used to sex Indian rhinoceros (Rhinoceros unicornis) samples.	en
dc.description.sponsorship	Objective 1—European Social Fund.	en
dc.identifier.citation	Peppin, L, McEwing, R, Ogden, R, Hermes, R, Harper, C, Guthrie, AJ & Carvalho, GR 2009, 'Molecular sexing of African rhinoceros', Conservation Genetics, doi:10.1007/s10592-009-9912-2. [http://www.springerlink.com/content/105709/]	en_US
dc.identifier.issn	1566-0621
dc.identifier.other	10.1007/s10592-009-9912-2
dc.identifier.other	7006535470
dc.identifier.other	J-6375-2013
dc.identifier.other	0000-0001-7729-9918
dc.identifier.uri	http://hdl.handle.net/2263/13758
dc.language.iso	en	en
dc.publisher	Springer	en
dc.rights	Springer. The original publication is available at www.springerlink.com	en
dc.subject	Molecular sex identification	en
dc.subject	Ceratotherium simum	en
dc.subject	Diceros bicornis	en
dc.subject	Zinc finger protein	en
dc.subject.lcsh	Polymerase chain reaction	en
dc.subject.lcsh	Rhinoceroses -- Sexing	en
dc.subject.lcsh	White rhinoceros -- Sexing	en
dc.subject.lcsh	Black rhinoceros -- Sexing	en
dc.title	Molecular sexing of African rhinoceros	en
dc.type	Postprint Article	en