Loop-mediated isothermal amplification (LAMP) detection of Babesia orientalis in water buffalo (Bubalus babalis, Linnaeus, 1758) in China

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dc.contributor.author He, Lan
dc.contributor.author Zhou, Yan-Qin
dc.contributor.author Oosthuizen, Marinda C.
dc.contributor.author Zhao, Jun-Long
dc.date.accessioned 2009-10-28T10:57:31Z
dc.date.available 2009-10-28T10:57:31Z
dc.date.issued 2009
dc.description.abstract Loop-mediated isothermal amplification (LAMP) is a rapid method with high specificity and efficiency under isothermal condition using a set of four specifically designed primers that recognize six distinct sequences on the target gene. In this study, a LAMPmethod was developed for specific detection of Babesia orientalis in water buffalo (Bubalus babalis, Linnaeus, 1758). Four primers were designed from the V4 hypervariable region of the 18S rRNA gene of B. orientalis. Blood samples were collected from B. orientalis experimentally infected water buffalo as well as from 165 water buffalo from eight different regions of the Hubei province, south China. Genomic DNA was extracted, subjected to the LAMP assay and compared with results obtained using a previously described semi-nested PCR. The LAMP assay proofed to be B. orientalis specific and more sensitive than the semi-nested PCR. While previously B. orientalis had not been reported north of the Yangtse River, our results show that B. orientalis has spread to the north of the river. This could pose a serious threat to the water buffalo industry. en
dc.identifier.citation He, L., et al., Loop-mediated isothermal amplification (LAMP) detection of Babesia He, L., et al., Loop-mediated isothermal amplification (LAMP) detection of BabesiaHe, L., et al., Loop-mediated isothermal amplification (LAMP) detection of Babesia orientalis in water buffalo (Bubalus babalis, Linnaeus, 1758) in China. Vet. Parasitol. (2009), doi:10.1016/j.vetpar.2009.06.036 en
dc.identifier.issn 0304-4017
dc.identifier.other 10.1016/j.vetpar.2009.06.036
dc.identifier.other 7004592997
dc.identifier.other N-8706-2014
dc.identifier.uri http://hdl.handle.net/2263/11643
dc.language.iso en en
dc.publisher Elsevier en
dc.relation.requires Adobe Acrobat Reader en
dc.rights Elsevier en
dc.subject Babesia orientalis en
dc.subject LAMP en
dc.subject 18S rRNA en
dc.subject Babesiosis en
dc.subject.lcsh Microbiological assay en
dc.subject.lcsh Babesiosis en
dc.subject.lcsh Veterinary protozoology en
dc.subject.lcsh Water buffalo en
dc.subject.lcsh Bubalus en
dc.title Loop-mediated isothermal amplification (LAMP) detection of Babesia orientalis in water buffalo (Bubalus babalis, Linnaeus, 1758) in China en
dc.type Postprint Article en


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