LOCUS Thielaviopsis_ethacetica_RNA_Virus_3 2991 bp RNA linear 23-JAN-2024 DEFINITION Thielaviopsis ethacetica RNA virus 3 strain JCM 6961. ACCESSION Thielaviopsis_ethacetica_RNA_Virus_3 VERSION KEYWORDS . SOURCE Thielaviopsis ethacetica RNA virus 3 ORGANISM Thielaviopsis ethacetica RNA virus 3 Unclassified. REFERENCE 1 (bases 1 to 2991) AUTHORS Hough,B., Wingfield,B. and Read,D. TITLE Identification and Characterization of Mycoviruses in Publicly available Transcriptomes from the fungal family Ceratocystidaceae JOURNAL unpublished REFERENCE 2 (bases 1 to 2991) AUTHORS Hough,B., Wingfield,B. and Read,D. TITLE Direct Submission JOURNAL Submitted (23-JAN-2024) Department of Biochemistry, Genetics, and Microbiology, University of Pretoria, Lynnwood Rd, Hatfield, Pretoria, Pretoria, Gauteng 0001, South Africa COMMENT Bankit Comment: TPA Submission:true Bankit Comment: TPA EVIDENCE:The viral sequences were derived from a publicly available transcriptome of the host fungus. This transcriptome is available on NCBI Sequencing Reads Archive (SRA). Contigs were assembled from this dataset by using the assembler rnaViralSPAdes, which is optimized for the assembly of RNA viruses. To identify putative mycoviruses, these contigs were converted to protein sequences, and were then aligned to a custom viral database consisting of viral proteins of various mycoviruses from various viral families. Once these contigs were identified, they were further analysed using BLASTp, to distinguish actual mycoviruses from any fungal derived proteins. This particular virus, Thielaviopsis ethacetica RNA virus 3, matched closely to members of the viral family Mitoviridae, and contained an ORF which encodes for an RdRp domain. Bankit Comment: ALT EMAIL:bientjie.hough@gmail.com Bankit Comment: TOTAL # OF SEQS:1 ##Assembly-Data-START## Assembly Method :: rnaViralSPAdes v. 3.15.1 Assembly Name :: Thielaviopsis_ethacetica_RNA_virus_3 Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..2991 /organism="Thielaviopsis ethacetica RNA virus 3" /mol_type="genomic RNA" /strain="JCM 6961" /host="Thielaviopsis ethacetica JCM 6961" /country="South Africa" /note="Viruses; Riboviria; Orthornavirae; Lenarviricota; Howeltoviricetes; Cryppavirales; Mitoviridae; Duamitovirus" CDS 410..2671 /codon_start=1 /transl_table=4 /product="RNA dependent RNA polymerase" /translation="MKESYSWQRGVKNLYHIRSQLLNIGRLIWGPTTITKVKGTMILL HRLESIRSRNGVPFLCKYMKSSAIYVMKYTASDPTSIHSHTYDIAISLTSSKLPRIIP REFREAIRMRDAHMIKWVLSIFNLYRVLPYVGKLKLSTITDPSGFSSLDGFQLWLSQQ EDYISSFKIRDKINPFLIETSGPCSPKGFNTTYGIFEALNTINVLFDFTKVLRPLLDP YGVYNDAKVGKWPIYKYFKSLSLLGQITSPIGMLSIKEEPGKLRVFAMLDVVTQWLLH PLHKAIFKFVSIFKYDATFDQQGTLDRFLNDHKDSSFWSFDLSAATDRLPIELQEQVL ATFVNPTFASAWRTILVERYYQLPKGKFNPKELILQYAVGQPMGAYSSWGMLDLTHHL IVKFAAHRVGKNVFDNYIILGDDVVIADEAVATSYYSIMTQELFVDINLSKGLISPNG FLEFAKRFKTYQDDYTPVSLREFSSWGSTYSSLFESIKNLKPSMFNLMRLFGRGSIAS GNNSNVLYKFLTIIHEILYVKTTDIIEFLSSLYPTLDSFFVRTFLINVIISESKIRKL KDLEKGTDRYNLRYGSQSNDRVVTASKLQLSSFGSSSLETVWKLGVTPETQAKINRVL SRSENDHDLYRLTDAQLMELYWLDLIPLQRTSNTNIFERLPAKENKSFLNSFKELFVI LPCIFDFVQYVHSNFPLINVASDKEEFISFIRPFIKTDVKIKISKRINFLYDPASKCM IPNVTEEQLPLKK" BASE COUNT 989 a 472 c 485 g 1045 t ORIGIN 1 ctccacctca cggtgttgta tttcctgaga cattgtctca gtcttcctta tatggaataa 61 ttaatttaat ttcatctaag aagatataac aatgagtatt tcattagtta atcatagagc 121 taccactaca tgatcttcga tctctcgcaa gatacatggc ataggggtta gcaccctcta 181 tatccgtggt gagattgcac gtagccacta ggttaactag gtggaacgca cgaaaggtaa 241 gaaattactt gtttgtgtaa accacaaata ccataactag tatgattatg aagtctgcct 301 gcggctcgaa taatattatt atcgaaaacc gaagtagaat agttatttct atctagagaa 361 cgctactaac aatacattaa ttaaattatt gcgagcatcg ctctttagaa tgaaagaatc 421 ttattcttgg caaagaggtg ttaaaaacct ctatcatata agaagccaat tattaaatat 481 tggacgtctt atctgaggac caaccacaat tactaaagtt aaaggtacta tgattttatt 541 acacagactc gaaagtataa gatcaaggaa cggtgttcct ttcttatgta aatatatgaa 601 atcttcagct atttatgtaa tgaaatacac tgcttctgat ccaacatcta ttcactctca 661 tacttacgat atagctatct ctcttacttc ttctaagtta ccaaggataa ttcctcgaga 721 atttagagaa gcaattagaa tgagagacgc tcatatgatt aaatgagtgt tatctatttt 781 taatctttat agagtattgc cctatgtagg aaagttgaaa ctgtctacaa taactgatcc 841 ttctggattt agttctttag atgggttcca attgtgatta tcacagcaag aagactatat 901 tagttctttt aaaattaggg ataagattaa tcccttttta attgaaactt ctggtccttg 961 ttctccgaag gggtttaata ctacatatgg tatctttgaa gctcttaata ctattaatgt 1021 attatttgat tttacaaaag tattaagacc tttattagac ccatatggtg tctataacga 1081 cgccaaagta ggtaaatgac ccatttataa atatttcaag agtttaagcc tccttggaca 1141 gattacatct ccaataggga tgttatctat caaagaggaa cctggaaaat taagagtctt 1201 tgctatgtta gacgttgtaa ctcaatgatt gttacatcct ctgcacaaag ccatatttaa 1261 atttgtaagt atatttaaat atgatgcgac ttttgatcaa cagggaactc ttgatcgttt 1321 cttaaatgat cataaagatt cttctttctg atcttttgat ttaagtgcgg ctactgatag 1381 attaccaatt gaactgcaag agcaggtatt agcaaccttt gttaatccta cttttgctag 1441 tgcatgacgt actatattag tagaaagata ctatcagtta ccgaaaggaa aatttaatcc 1501 taaagaatta attcttcagt atgctgtggg tcaacctatg ggagcttata gctcttgagg 1561 aatgttagat ttaactcatc atttgatagt taaatttgct gcccacagag taggtaaaaa 1621 cgtttttgat aattatatca tattgggaga tgatgtagtt atagctgatg aagctgtagc 1681 tacttcttac tactcaataa tgactcaaga attatttgtt gatattaatc tttctaaagg 1741 attaatttct ccaaatggtt tcctagagtt tgctaaacgt tttaaaactt atcaagatga 1801 ttatactcct gtatctttac gggaattctc atcttgaggg tctacttata gttccctttt 1861 cgaaagtatt aaaaatttaa aaccttctat gttcaatctt atgagattgt tcggtagggg 1921 ttctatagct agtggtaaca attctaatgt attatataaa tttcttacta taattcatga 1981 aatactgtac gtaaaaacta cagatattat tgaattttta agttctttat atcctacttt 2041 agatagtttc tttgttagaa cctttcttat aaatgtgata atctctgaat ctaaaattcg 2101 gaaattaaag gatttagaga aaggaactga ccgttataat ttgagatacg gatcacaatc 2161 taatgataga gttgtgactg cttccaaatt acaattgtca tctttcggat cgtcatcttt 2221 agagacggtc tgaaagttgg gagtaactcc agaaactcaa gcaaaaatta atcgagtatt 2281 atcaagatca gaaaatgatc atgatttata ccgtttaaca gatgcccaac taatggaatt 2341 atactgatta gatttaatac ctcttcaacg tacttcaaat acaaatatct ttgaaagatt 2401 accggctaaa gaaaataaat ctttccttaa ttcttttaag gaattatttg taatcttacc 2461 ttgtattttc gattttgttc aatatgttca tagtaacttt cccttaatta atgtggcctc 2521 agataaggaa gaatttattt cttttattag accatttatc aaaactgatg ttaagataaa 2581 gatctctaaa cgaataaatt tcttatatga cccagcgtct aagtgtatga ttccgaatgt 2641 aactgaagaa cagttacctt tgaaaaaata atttagatta attaatatgg ttgtaccatg 2701 ttctaatcaa acattggaca gtatatagtt ataccatctt ttactatatc gtaaataatt 2761 ggtataaata gccaaatgta ctagaagact ccgccttaaa caagcgctgt gaagtttagt 2821 attctgtata tgttaaaacc ctctattaat tcttataaat ttgaattata ggcttagatg 2881 aataaaagta aaattatctc atctaagaga gctctgtaga aatacaggga aatacctacc 2941 tgtgagctgt ctggccgtgc ctcgatgagt tggcaataag tcgcttgtcg t //