LOCUS Thielaviopsis_ethacetica_RNA_Virus_2 2616 bp RNA linear 23-JAN-2024 DEFINITION Thielaviopsis ethacetica RNA virus 2 strain JCM 6961. ACCESSION Thielaviopsis_ethacetica_RNA_Virus_2 VERSION KEYWORDS . SOURCE Thielaviopsis ethacetica RNA virus 2 ORGANISM Thielaviopsis ethacetica RNA virus 2 Unclassified. REFERENCE 1 (bases 1 to 2616) AUTHORS Hough,B., Wingfield,B. and Read,D. TITLE Identification and Characterization of Mycoviruses in Publicly available Transcriptomes from the fungal family Ceratocystidaceae JOURNAL unpublished REFERENCE 2 (bases 1 to 2616) AUTHORS Hough,B., Wingfield,B. and Read,D. TITLE Direct Submission JOURNAL Submitted (23-JAN-2024) Department of Biochemistry, Genetics, and Microbiology, University of Pretoria, Lynnwood Rd, Hatfield, Pretoria, Pretoria, Gauteng 0001, South Africa COMMENT Bankit Comment: TPA Submission:true Bankit Comment: TPA EVIDENCE:The viral sequences were derived from a publicly available transcriptome of the host fungus. This transcriptome is available on NCBI Sequencing Reads Archive (SRA). Contigs were assembled from this dataset by using the assembler rnaViralSPAdes, which is optimized for the assembly of RNA viruses. To identify putative mycoviruses, these contigs were converted to protein sequences, and were then aligned to a custom viral database consisting of viral proteins of various mycoviruses from various viral families. Once these contigs were identified, they were further analysed using BLASTp, to distinguish actual mycoviruses from any fungal derived proteins. This particular virus, Thielaviopsis ethacetica RNA virus 2, matched closely to members of the viral family Mitoviridae, and contained an ORF which encodes for an RdRp domain. Bankit Comment: ALT EMAIL:bientjie.hough@gmail.com Bankit Comment: TOTAL # OF SEQS:1 ##Assembly-Data-START## Assembly Method :: rnaViralSPAdes v. 3.15.1 Assembly Name :: Thielaviopsis_ethacetica_RNA_Virus_2 Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..2616 /organism="Thielaviopsis ethacetica RNA virus 2" /mol_type="genomic RNA" /strain="JCM 6961" /host="Thielaviopsis ethacetica JCM 6961" /country="South Africa" /note="Viruses; Riboviria; Orthornavirae; Lenarviricota; Howeltoviricetes; Cryppavirales; Mitoviridae; Unuamitovirus" CDS 400..2424 /codon_start=1 /transl_table=4 /product="RNA dependent RNA polymerase" /translation="MKSVRLHITRYMCGKPLKSNTDRVSLKNNFPTRFYYLKEILDSK NKVKIRGVLTLLYFTRALIPTKAEEEKIKVDLSTITSNYKGKDYSIPMWFINLFVQKN KLNQPKPVFDNSLHYISSKGSPFGKATISGPYALFNKAMAHHSMLNHYLALIGEKCYK LIFGNFIQEIVKDNRLFTITRFSDSLGKLSIVKDPECKRRVIAMLDYNSQLLLRPIHD ALLRSLTKLPCDRTYTQDPVHKWSPNGNQFWSLDLSAATDRFPIKLQTKVISSIFGDR AFAEAWEKILIDRNFNYNDQSVRYSVGQPMGAYSSWAAFTVSHHLVVAWCAHLCKISD FNDYIILGDDIVIRNDKVAQKYISIMTKLGVDISLNKTHTSKNCYEFAKRWIINGIEV SPLPLRGILSNVNKPLVVLQQLMIYLQRNNTIFNGNSLQLISEIYNGIKIKNRVFTSN SIYKYCYDFYHILRYAYGLSSNNEIRNYLVMKRIPDVFLVNEKLIPSFMKALLLLGLS GAAEKAGNEIKTWFEDFIKPYKEFDNFQISDLKNHYLTHALYNRAYSISKEIQKANRD QNFDLLDCMNSMRLEKIDKITSLHRDKSTTVSNLDKLWKSSISKLKLINDENFYNFPI SQFDQLYDLKPWENHFLQNLSVSKDALDVLRYGTYNDPNKPAAFDYSCFI" BASE COUNT 1004 a 359 c 359 g 894 t ORIGIN 1 acaacctaaa gttgtaggga aaacggatct ttgatcctat acacttaaag attgatcttt 61 aactgtatac gtttaattat aagcctttaa tttccaaatt gaaggaccta ttataatgat 121 aagttttact ttgatatact gattaattat ccaacctagg ttagtcacct acaaagatat 181 taatcttcac tgttatatta aggtatagat caatttatca ttatattata aaataatata 241 atatatctga ctactaacca acaaaacaaa gtaacatata caataaccat cgggttatta 301 catattctat ttggaataga caaaactgtt acaaagagat ttctttatag tattcaatct 361 ttaagggata aaagtgggtt actatacaca atcaagtata tgaaatcggt gagattacat 421 attacaagat acatgtgtgg taaacctctt aaatctaata cagatagagt atctttaaag 481 aataattttc ctactcgttt ttattatcta aaagaaattt tagatagtaa aaataaagtt 541 aaaattagag gtgttttaac acttctatat tttacaagag cgctaattcc aacgaaagct 601 gaagaggaaa agataaaagt tgatttatca actattacct caaattacaa aggaaaagat 661 tactcaattc cgatgtgatt tattaattta tttgttcaaa agaataaatt aaatcaaccc 721 aaaccagtct ttgataactc acttcactac attagtagta aagggagccc atttggtaaa 781 gcaactatct ctggtccata tgccttattt aataaagcaa tggctcacca ttcaatgtta 841 aatcattatt tagcattaat tggtgaaaag tgttataaat taatatttgg taactttata 901 caagaaattg ttaaagataa cagattattt actataacaa gattcagtga cagtttaggt 961 aaattatcta tagtaaaaga tcctgaatgt aaaagaagag ttatagccat gttagactat 1021 aattctcaat tactgttacg tcctatacat gatgctttat taagatcatt aacaaagcta 1081 ccatgtgata gaacatatac acaagatcca gtacataaat gatctccgaa tggtaatcaa 1141 ttttgatcac tagatttgtc ggccgcaaca gatagatttc caatcaaact tcaaacaaaa 1201 gttatctctt caatttttgg agatagagct tttgctgaag catgagagaa aatcctaatt 1261 gatcgtaatt ttaattacaa tgatcaaagt gtaagatata gtgtaggtca acccatggga 1321 gcatatagct cttgagctgc cttcactgta tctcatcacc tagttgtagc ttgatgtgca 1381 catttatgta aaatatcaga ttttaatgac tacatcatct taggtgatga catagtaata 1441 agaaatgata aagtagcaca aaagtacata tcaattatga ctaaattagg agtggatata 1501 tctttaaaca aaactcatac aagtaaaaat tgttatgaat ttgccaaaag atgaataatc 1561 aatggtatcg aagtaagtcc tttaccatta agaggaatat tatctaatgt taataaacca 1621 ttagttgtct tacaacaatt aatgatttat ttacaaagaa ataatacaat tttcaatgga 1681 aatagcttac aattaatatc agaaatttat aatggtatta aaataaagaa tagagttttt 1741 acctctaatt ctatatataa atactgttat gatttctatc atatactaag atatgcatat 1801 ggattatctt caaataacga aataagaaat tatcttgtta tgaaaagaat tccagatgtt 1861 ttcttagtca atgaaaagtt aattccttca tttatgaagg cactactatt gttaggatta 1921 tctggtgccg ctgagaaagc aggtaatgaa attaaaacct gatttgagga ttttattaaa 1981 ccttataaag aatttgataa tttccaaata tcagatctta aaaatcatta tctaactcat 2041 gcattataca atcgtgctta tagcatttcc aaggaaatac aaaaagcaaa tagagatcaa 2101 aattttgacc tattagattg tatgaattct atgagactag aaaagataga taagattact 2161 tctcttcaca gagataaatc tacaactgtc tcaaatttag ataaattgtg aaaaagtagt 2221 atttcaaaat taaaattaat taatgatgaa aatttctaca atttcccaat tagtcaattt 2281 gatcaattat atgatctaaa accatgagaa aatcatttct tacaaaatct ttcagtatca 2341 aaagatgcac tagatgtact aagatatggt acttataatg atcctaataa accagctgca 2401 tttgattaca gctgttttat ataggagatc ttaagctatt gttatccttt agataatata 2461 tttctcaaga ttatatcatt tgtacagtgg agttttctat tctataatta atattatgag 2521 agtaatggtt actctctttc attaataata ttaattaagg aatatgtaat actgtggttt 2581 agaaaccacc tttcttcctg agcttttaca gcttag //