LOCUS Thielaviopsis_ethacetica_RNA_virus_1 2620 bp RNA linear 23-JAN-2024 DEFINITION Thielaviopsis ethacetica RNA virus 1 strain JCM 6961. ACCESSION Thielaviopsis_ethacetica_RNA_virus_1 VERSION KEYWORDS . SOURCE Thielaviopsis ethacetica RNA virus 1 ORGANISM Thielaviopsis ethacetica RNA virus 1 Unclassified. REFERENCE 1 (bases 1 to 2620) AUTHORS Hough,B., Wingfield,B. and Read,D. TITLE Identification and Characterization of Mycoviruses in Publicly available Transcriptomes from the fungal family Ceratocystidaceae JOURNAL unpublished REFERENCE 2 (bases 1 to 2620) AUTHORS Hough,B., Wingfield,B. and Read,D. TITLE Direct Submission JOURNAL Submitted (23-JAN-2024) Department of Biochemistry, Genetics, and Microbiology, University of Pretoria, Lynnwood Rd, Hatfield, Pretoria, Pretoria, Gauteng 0001, South Africa COMMENT Bankit Comment: TPA Submission:true Bankit Comment: TPA EVIDENCE:The viral sequences were derived from a publicly available transcriptome of the host fungus. This transcriptome is available on NCBI Sequencing Reads Archive (SRA). Contigs were assembled from this dataset by using the assembler rnaViralSPAdes, which is optimized for the assembly of RNA viruses. To identify putative mycoviruses, these contigs were converted to protein sequences, and were then aligned to a custom viral database consisting of viral proteins of various mycoviruses from various viral families. Once these contigs were identified, they were further analysed using BLASTp, to distinguish actual mycoviruses from any fungal derived proteins. This particular virus, Thielaviopsis ethacetica RNA virus 1, matched closely to members of the viral family Mitoviridae, and contained an ORF which encodes for an RdRp domain. Bankit Comment: ALT EMAIL:bientjie.hough@gmail.com Bankit Comment: TOTAL # OF SEQS:1 ##Assembly-Data-START## Assembly Method :: rnaViralSPAdes v. 3.15.1 Assembly Name :: Thielaviopsis_ethacetica_RNA_virus_1 Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..2620 /organism="Thielaviopsis ethacetica RNA virus 1" /mol_type="genomic RNA" /strain="JCM 6961" /host="Thielaviopsis ethacetica JCM 6961" /country="South Africa" /note="Viruses; Riboviria; Orthornavirae; Lenarviricota; Howeltoviricetes; Cryppavirales; Mitoviridae; Unuamitovirus" CDS 341..2587 /codon_start=1 /transl_table=4 /product="RNA dependent RNA polymerase" /translation="MKYTPQLIIERLINLIFNINGSMYVSRYLLLFNSMRKNNGLLYT IKWFKAAKLHITRYICGKPLLSNTEGVSVDKEGFPTRLDFLKPLVKENPRALLTLLSF TRSVEPTKEEEKYRKPKTDSIVAPYKGKEYTIPKSFIESWIKQFDLNLEKHSYSIKDH YLSMKGSPNGKSSISSLWSLAFHSMDTLGYIKYLCGDFYNQLSKVYINLWVHHRDLLE KKATKNTIGKLSVVHDPELKERVIAMVDYTTQFILRPIHDDLLSLLKKLPCDRTFTQS PFHDWLDSNDCYYSLDLSSATDRFPIQLQQKLIALIYKDYEFARNWKDLLVDRSYWFE SKEYRYSVGQPMGAYTSWAAFTLTHHLVVAWAAKLAGYSTFHDYIILGDDIVIHNDRV AQRYINLMNKFGVEISVPKTHVSRNTYEFAKRWIQNGKEITGLSLRGILINIKHIHVV YMNIFTYLQRIPSLNVNVLQIVCKLYTNVKIGNRIKTSNSIFRSLYDFHHSIRYSFGL LTYEELRNYLINKIPFEEFIPWPMGSAHSRVNEILSEGIVDQAKALNSKLKSEFFKLE DFDLSSELMKTWPLTYGYHNHIQKLKSSLLSLVNGSTDLIDIISEVRLQNIDSILKFK RNTFENLILLDKLWKKAFKVYKDKLSKELVPKEENPYAPVGGLVQHIWGIPKSSPFTP YKSTHFGLKTWKEELETNINSLDRDLDFFFNAEVQPEHKEHIQDRIIAYVIDCKRKGM LPKFDDFK" BASE COUNT 939 a 415 c 415 g 851 t ORIGIN 1 tcctacatgg gataaaaggg cgagacgaaa cttgccataa ctaagtttta agttagttat 61 ccagaatctt tgttcacaaa tgaagattca aagaatagaa accagcaatg gacttctcta 121 ttatatgtgg agtagctttc tagtaataga tcgccaagtc tacatgtata catgttacgt 181 aaatttagta ggattgatca tcctttgata aagtttacgt cccatatagg acagtagtgg 241 catcagatgt ccgtccatat gaagatatag atgaactata tttagagtta aaagctcctg 301 atatagagaa tatatgatct tataattaat aacaatcaac atgaaataca caccacaact 361 aataattgag agactaatta acttaatctt caatattaat ggtagtatgt atgtgtcacg 421 ttatttattg ctctttaata gtatgagaaa gaataacgga ttactatata caatcaaatg 481 atttaaagcc gcaaagcttc atatcacaag atatatatgt ggtaaaccat tattaagtaa 541 tacagaagga gtttctgttg acaaagaggg gtttcctacg cgtttagatt ttcttaaacc 601 tttagttaaa gaaaatccaa gagcgcttct aactttatta agttttacac gtagtgtaga 661 acctactaag gaagaagaga aatatagaaa accaaagact gattctattg tagcacccta 721 taaaggaaaa gagtatacta tacccaaatc ttttatagaa tcctgaataa aacagtttga 781 tctaaatctt gagaaacatt catatagtat taaagaccat tacctaagta tgaaaggttc 841 tcctaacgga aaatcttcta tatctagttt atgatcttta gctttccact caatggacac 901 acttgggtat ataaaatacc tttgtggtga tttctataac caactttcga aggtttatat 961 aaatctttga gttcaccata gggatcttct tgaaaagaag gccactaaga atactattgg 1021 aaaactatct gtagtacatg accctgagtt aaaagaaaga gtaattgcta tggtagacta 1081 taccacacaa tttattcttc gtcctataca tgatgatctc ttaagtttac ttaagaaatt 1141 accttgtgat aggactttta ctcaaagtcc tttccatgac tgattagaca gcaatgattg 1201 ctactattca cttgaccttt cttctgcaac tgatagattt cctatacaat tacaacaaaa 1261 attaatagcc ttgatttata aagactatga atttgcgcgt aattgaaagg acttacttgt 1321 agatcgttcc tactggtttg agtcaaaaga atatcgctat tctgttggtc aaccaatggg 1381 agcttatact agttgagctg ctttcacatt aacacatcat cttgttgtag catgagctgc 1441 taaattagca ggttattcta cttttcacga ttatataatc ctaggtgacg atatcgtaat 1501 acataacgat agagttgcac aaagatatat aaacttgatg aacaagttcg gtgttgaaat 1561 cagtgttcca aaaactcatg taagtagaaa tacttacgag tttgcgaaac gttgaattca 1621 aaatggaaaa gaaatcactg gtctatcttt aagaggaatt cttataaata ttaaacatat 1681 tcacgtagta tacatgaaca tatttacata tttacaaaga attccttctt taaatgtgaa 1741 cgtcttacag attgtatgta aattatatac taatgtaaag atcggaaata gaattaaaac 1801 ttctaattca atctttagat cattatatga tttccatcat tctataagat actcatttgg 1861 ccttcttact tacgaggagt tgcgaaacta cttgataaat aagataccat ttgaggagtt 1921 tattccatga cctatgggat cagctcattc acgagtgaat gaaatcttgt cagagggaat 1981 agttgaccaa gctaaagcct taaactcaaa attaaaatca gaattcttca aacttgaaga 2041 ttttgatctt agttctgaac ttatgaagac atgaccttta acatatggtt atcataacca 2101 tatacaaaag ttaaaatctt cattactttc attagtaaat ggatctacag atttaataga 2161 tattatttct gaagtccgtc tacaaaatat agatagtatt ttgaaattca aacgtaatac 2221 atttgaaaat ctaatacttc tagataaatt atgaaagaag gcttttaagg tatataagga 2281 taaactttct aaggaacttg ttccaaaaga agaaaatcct tatgcacctg tgggtggatt 2341 agtacaacat atatggggaa ttcctaagag ttcaccattt acaccttaca aatctactca 2401 ctttggtctg aagacatgaa aagaagaatt agagaccaat atcaattcac ttgataggga 2461 tcttgacttc ttttttaatg ccgaagttca acctgagcat aaagaacaca tacaagatcg 2521 tattatagcc tatgtcatag actgtaaacg aaaaggtatg ctacctaaat ttgatgattt 2581 taagtaatta tctctggagt taaactccct ttttatcccg //