LOCUS Huntiella_omanensis_RNA_Virus_1_ORF2 2322 bp RNA linear 23-JAN-2024 DEFINITION Huntiella omanensis RNA Virus 1 strain CMW44450. ACCESSION Huntiella_omanensis_RNA_Virus_1_ORF2 VERSION KEYWORDS . SOURCE Huntiella omanensis RNA Virus 1 ORGANISM Huntiella omanensis RNA Virus 1 Unclassified. REFERENCE 1 (bases 1 to 2322) AUTHORS Hough,B., Wingfield,B. and Read,D. TITLE Identification and Characterization of Mycoviruses in Publicly available Transcriptomes from the fungal family Ceratocystidaceae JOURNAL unpublished REFERENCE 2 (bases 1 to 2322) AUTHORS Hough,B., Wingfield,B. and Read,D. TITLE Direct Submission JOURNAL Submitted (23-JAN-2024) Department of Biochemistry, Genetics, and Microbiology, University of Pretoria, Lynnwood Rd, Hatfield, Pretoria, Pretoria, Gauteng 0001, South Africa COMMENT Bankit Comment: TPA Submission:true Bankit Comment: TPA EVIDENCE:The viral sequences were derived from a publicly available transcriptome of the host fungus. This transcriptome is available on NCBI Sequencing Reads Archive (SRA). Contigs were assembled from this dataset by using the assembler rnaViralSPAdes, which is optimized for the assembly of RNA viruses. To identify putative mycoviruses, these contigs were converted to protein sequences, and were then aligned to a custom viral database consisting of viral proteins of various mycoviruses from various viral families. Once these contigs were identified, they were further analysed using BLASTp, to distinguish actual mycoviruses from any fungal derived proteins. The genome segment of this particular virus, Huntiella omanensis RNA Virus 1, matched closely to members of the viral family Totiviridae, and contained an ORF which encodes for an RdRp domain. Bankit Comment: ALT EMAIL:bientjie.hough@gmail.com Bankit Comment: TOTAL # OF SEQS:1 ##Assembly-Data-START## Assembly Method :: rnaViralSPAdes v. 3.15.1 Assembly Name :: Huntiella_omanensis_RNA_Virus_1_ORF2 Sequencing Technology :: IonTorrent ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..2322 /organism="Huntiella omanensis RNA Virus 1" /mol_type="genomic RNA" /strain="CMW44450" /host="Huntiella omanensis CMW44450" /country="South Africa" /note="Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; Victorivirus" CDS 42..2192 /codon_start=1 /product="RNA dependent RNA polymerase" /translation="MSPGSIAELFPHKNDTAAINKNSVSLTRLVAAIRRTHDPRPITE LLRVGPGVWSNDQACSFLLYQAALTPTLGARGLRYASSIVASPADAKGLSNALKALGA NSCRVGALLTEGQNLQGRGVGGGKLREEFDYRTDPVAIKEKVLSLSAEELRPHVRAII SAELTTKPDFEPVDVWWSKRWKWCVNGTQNALSNQALKLDSSKWTRTHARAYRKMASE GVELEPTATWDGVTLVSVAPKLEHGKTRTIFACDTLSYYAFARLLDPVQKVWRNRRVL LDPGSGGMSGIVRRIRRAQVGGGVNLMLDYDDFNSQHSTEVMAMVFDELCRLTDYPPE AAAVLVRSFSTTYISFDGELRRSMGTLMSGHRGTTFINSVLNAAYIRASVGGPPFDAM TSFHTGDDVYINSPTFSESLRILRSCRATGLRMNPSKQSLGRHSAEFLRMAIGKSCAF GYLPRAIASLTCGNWVVPDALSPDEALRSLISTLRSVINRSGNTAIPYLVARSVRSSG LSGISLSVLTDLFTGRAALAGSPVFGGAPQVVTYSMGVPPPIDEPPDPAWPTYASRAY LRDHVSLVEREALAMTKVDPLPTLVNLSYSKGRTRVKAQGSVPVVLAPAKFTKVTGTA LVHELLDTPQKEGCLSKFPLVNLVSQRLNRSQLAELVLLAGGDPLAPNLEKEAFGAEA VTHRIVGCLPYSDAATLSKRTTKGTILAGFPVCM" BASE COUNT 417 a 789 c 592 g 524 t ORIGIN 1 actaccgaac cctgaggatt ccgtcccgtc gttgtaagag aatgagcccc ggttccatcg 61 ccgagctctt tcctcacaag aacgacaccg ccgcgataaa caagaactcg gtcagcctta 121 cccgcctcgt agctgccatc cggcggaccc acgacccccg ccctatcacg gaactcctcc 181 gcgtaggtcc cggcgtctgg tccaacgatc aagcgtgttc tttcctgctg taccaagcag 241 ccctcacccc cacccttggt gcgcgaggcc tccgctatgc ctcatccatt gttgcgtctc 301 ctgctgacgc caaaggatta tccaacgccc tcaaagctct gggcgctaac tcttgtcggg 361 ttggtgccct cttaactgag ggccagaacc tgcagggccg tggcgtcggt ggcggtaagc 421 tccgagaaga gttcgattac cgcaccgacc ctgtcgcgat caaagagaag gttctctcgc 481 tctcggctga ggagctccgc cctcacgtcc gagctatcat ctccgcggag ctgacaacga 541 agcctgactt cgagcctgtc gacgtatggt ggtcaaaacg ctggaagtgg tgcgttaacg 601 gtacccagaa cgccctttcc aaccaggccc tgaagttaga ctcgagtaag tggactcgca 661 ctcatgcccg ggcctatcgc aagatggctt ccgagggtgt tgagttggaa cccaccgcca 721 cctgggatgg cgttaccctc gtctcagtcg ctcccaaatt ggagcatggg aagacgcgca 781 caatttttgc ttgtgatacg ctctcctact atgccttcgc ccgccttctt gaccccgtcc 841 agaaggtctg gcgcaaccga cgtgtcctcc tagacccggg tagtggtggg atgtccggga 901 ttgtccggcg catccgccgt gcccaggtcg gaggcggtgt caacttaatg ctggactacg 961 acgacttcaa ttcccagcac tccacagagg tgatggccat ggtctttgac gagctgtgtc 1021 gcctcactga ctaccctccg gaggctgccg ccgtgcttgt tcgctcgttc tccacaacct 1081 atatctcctt cgacggtgaa ttgcgtcgtt cgatgggcac tctaatgagt ggccatcgtg 1141 ggacgacgtt cattaactcc gtcctcaacg ccgcctacat tcgtgcgtct gtaggtggcc 1201 cccccttcga tgcgatgacg tcattccaca ctggtgacga cgtctacatc aactctccca 1261 ccttctccga atccctacgg attctacgct cctgccgtgc cactggtctc cggatgaacc 1321 catccaagca gagcctgggc cgccattccg ccgagttcct ccggatggcg atcggcaaat 1381 cttgtgcttt cgggtacctc cctcgcgcaa ttgcttcgct cacgtgtggc aattgggtcg 1441 tacccgacgc cctctcgccg gatgaggcct tgcgcagcct catttcgaca cttcgttcgg 1501 tcatcaaccg ctctggtaac acggcgatcc cctacctcgt tgcacggtcc gtccgttcct 1561 ccgggttgag tggcatcagc ctgtctgttc ttacagactt gttcaccggt cgtgccgccc 1621 ttgccgggag ccccgttttt ggcggtgccc cccaggtcgt cacgtactct atgggcgtgc 1681 ccccccctat tgatgaaccc cccgaccccg cttggccgac ctacgcctcc cgggcgtatc 1741 ttagggacca cgtttctctc gtcgaaagag aggctctagc gatgacgaag gtagacccgc 1801 tgcccaccct agtgaactta tcctactcca aaggccgcac tagggtcaag gcacaggggt 1861 ctgtaccagt cgttcttgct cctgccaaat tcacgaaagt tactggaacc gctctcgtcc 1921 acgagctgct cgatacgccg caaaaagaag gctgtctctc caagttccct ctcgtcaacc 1981 ttgtcagcca gaggttgaat cggtcccaac tggctgaact tgtcctcctc gccggaggcg 2041 acccccttgc tcctaacctc gaaaaagagg cattcggagc tgaggcggtc acccaccgca 2101 tcgtcggctg cctgccctac tccgacgctg cgacgctttc caagcgtact accaagggta 2161 ccattctcgc tggcttcccg gtgtgcatgt agacacctac cgtgtacacc gccagccctc 2221 ccccactcct acgtttgcct tgttagggcg atcgtaggag gttctttatc cgtcccccgc 2281 aagggggaca aagcagtctc tggttcgctt gccttcgcca cc //