LOCUS Huntiella_omanensis_RNA_Virus_1_ORF1 1852 bp RNA linear 23-JAN-2024 DEFINITION Huntiella omanensis RNA Virus 1 strain CMW44450. ACCESSION Huntiella_omanensis_RNA_Virus_1_ORF1 VERSION KEYWORDS . SOURCE Huntiella omanensis RNA Virus 1 ORGANISM Huntiella omanensis RNA Virus 1 Unclassified. REFERENCE 1 (bases 1 to 1852) AUTHORS Hough,B., Wingfield,B. and Read,D. TITLE Identification and Characterization of Mycoviruses in Publicly available Transcriptomes from the fungal family Ceratocystidaceae JOURNAL unpublished REFERENCE 2 (bases 1 to 1852) AUTHORS Hough,B., Wingfield,B. and Read,D. TITLE Direct Submission JOURNAL Submitted (23-JAN-2024) Department of Biochemistry, Genetics, and Microbiology, University of Pretoria, Lynnwood Rd, Hatfield, Pretoria, Pretoria, Gauteng 0001, South Africa COMMENT Bankit Comment: TPA Submission:true Bankit Comment: TPA EVIDENCE:The viral sequences were derived from a publicly available transcriptome of the host fungus. This transcriptome is available on NCBI Sequencing Reads Archive (SRA). Contigs were assembled from this dataset by using the assembler rnaViralSPAdes, which is optimized for the assembly of RNA viruses. To identify putative mycoviruses, these contigs were converted to protein sequences, and were then aligned to a custom viral database consisting of viral proteins of various mycoviruses from various viral families. Once these contigs were identified, they were further analysed using BLASTp, to distinguish actual mycoviruses from any fungal derived proteins. This particular virus, Huntiella omanensis RNA Virus 1, matched closely to members of the viral family Totiviridae, and contained an ORF which encodes for an partial coat protein domain. Bankit Comment: ALT EMAIL:bientjie.hough@gmail.com Bankit Comment: TOTAL # OF SEQS:1 ##Assembly-Data-START## Assembly Method :: rnaViralSPAdes v. 3.15.1 Assembly Name :: Huntiella_omanensis_RNA_Virus_1_ORF1 Sequencing Technology :: IonTorrent ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1852 /organism="Huntiella omanensis RNA Virus 1" /mol_type="genomic RNA" /strain="CMW44450" /host="Huntiella omanensis CMW44450" /country="South Africa" /note="Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; Victorivirus" CDS 309..>1852 /codon_start=1 /product="Coat Protein" /translation="MDRVETPSSYLTGVLASPRGGYIASAAFRRYTSAVTTSATVRGV LDSRITAIRYEVGRGVESVKDALAPASSTLGLGVSASYATSSVLAEDFMGFAKKYSNF SATFEFSNLAGVAERLARGLAACSVFKGVDSGALAGGNDISISALGAHSAPVNTTLTS VFVPRLVSSVLAPDVFGVLIAAVAGEGSSIVTDVLALDEDDKPIIPAADEFAFAHACV EALRIVGANMIAADQGPLFALAVTRGIHRVVSVVGHTDEGGILRDVLRCSHFAPPFGG IHYGLTDYTGLPGLSSSSVASVAAFVDTVALVTAALVAHCDPGVVYDGMWFPTVIEGT SVSDVASEPGADYPGADRHATRNRAQLLAIIGEFGSTYVRGLGVIFGAAGHTDTALRV FTAAASRLGATPRHLKHPSVAPYFWVEPTSLLPPRLLDTPAEQNGAGSIAYRDSTRSL QAWEDAYQVGEVGPSFSTNVVTFRGARYAPLLFHWLGHPENGLGAIRPRQMDPAQIVH PGRRALR" BASE COUNT 256 a 683 c 483 g 430 t ORIGIN 1 tgtcctcgcc ttcctccccc tttctgccaa gcgaaccgac acctgtcgga tctcgcggcc 61 acgggggtgg ttatttggac gtttggcgcg gtaatgtcac agccttccgg ctctcccatg 121 tcggatagag gcacctcttg aataaagagt cctgacctcg acaaggtggt tgtctagtct 181 gattagggag ggaggagtag cgagagctac tctccgttga tctaatcgtc tagattgcta 241 acgtgatatt gttccgctgc gcctcgtcca aaaaccattt tttctaaaac cccgctcccg 301 tcctcgaaat ggaccgtgtc gaaactcctt cgtcttacct cacgggtgtc ctcgcctccc 361 cccgaggtgg gtacattgcc tccgctgcct tccgccgcta cacctcggct gtcacgacct 421 ctgctaccgt ccgcggagtc ctcgattcgc gtatcaccgc gattcgttac gaagtcggtc 481 gtggcgttga gtctgtcaag gacgccctcg ccccagcttc ctccacgctt gggctaggcg 541 tatccgcgtc gtacgccacc tcctccgtcc tcgctgagga tttcatgggc ttcgcaaaga 601 agtactcgaa cttctccgcc accttcgagt tttccaacct tgccggtgtc gctgaacgtc 661 tcgcccgcgg cttggccgcc tgttctgttt ttaagggcgt tgactctggc gctctcgctg 721 gagggaatga tatttccatc tccgctcttg gagcccactc ggcccccgtc aacaccaccc 781 tcacgtcggt gttcgtcccc cgcctcgtct cgagcgtcct tgcccccgac gttttcggtg 841 ttctcatcgc cgccgtcgcc ggtgagggca gctctatcgt caccgacgtc ctcgccttgg 901 atgaagacga caaaccgatc atccccgccg ctgacgagtt cgccttcgcc cacgcctgtg 961 tcgaggctct tcgcatcgtc ggcgctaaca tgattgctgc ggaccagggt cccctcttcg 1021 ccctcgctgt cacccgcggc atccaccgag tcgtctccgt cgtcggccac actgacgagg 1081 gcgggatcct acgtgatgtc ctccgctgta gccacttcgc ccctcccttc ggtggaatcc 1141 actacgggtt gaccgattac actggcctcc caggcctctc ctcttccagc gttgcctctg 1201 tggccgcttt cgtggatacg gttgccttgg tcactgcggc gcttgttgcc cactgtgacc 1261 ctggcgtcgt ctatgacggt atgtggttcc cgactgtgat cgagggtacc tccgtgtcgg 1321 acgtcgcttc tgagcccggt gccgactacc ccggcgctga tcgccatgcc acccgcaacc 1381 gtgcccagct gctcgccata atcggtgagt tcggctcgac ctacgtccgt ggcctgggcg 1441 tcatcttcgg cgcggcgggc cacactgaca ccgccctccg cgtcttcacc gcggctgctt 1501 ctcgcctcgg cgccaccccg cgccacctca aacacccttc cgtcgctccc tacttctggg 1561 ttgagccgac gtccctcctc cccccccggc ttcttgacac ccccgccgag cagaacggtg 1621 ccgggtcgat cgcttaccgc gactccaccc gctcccttca ggcttgggag gacgcttacc 1681 aggtcggtga ggtcggcccc tctttctcca cgaacgtcgt cactttccgc ggagcccggt 1741 acgctcccct cctcttccac tggctcgggc accctgagaa cgggctcgga gccatccgcc 1801 cccggcagat ggaccccgcc cagattgtcc accctggtcg ccgggccctc cg //