LOCUS Thielaviopsis_paradoxa_RNA_Virus_1 2599 bp RNA linear 23-JAN-2024 DEFINITION Thielaviopsis paradoxa RNA Virus 1 strain T-189. ACCESSION Thielaviopsis_paradoxa_RNA_Virus_1 VERSION KEYWORDS . SOURCE Thielaviopsis paradoxa RNA Virus 1 ORGANISM Thielaviopsis paradoxa RNA Virus 1 Unclassified. REFERENCE 1 (bases 1 to 2599) AUTHORS Hough,B., Wingfield,B. and Read,D. TITLE Identification and Characterization of Mycoviruses in Publicly available Transcriptomes from the fungal family Ceratocystidaceae JOURNAL unpublished REFERENCE 2 (bases 1 to 2599) AUTHORS Hough,B., Wingfield,B. and Read,D. TITLE Direct Submission JOURNAL Submitted (23-JAN-2024) Department of Biochemistry, Genetics, and Microbiology, University of Pretoria, Lynnwood Rd, Hatfield, Pretoria, Pretoria, Gauteng 0001, South Africa COMMENT Bankit Comment: TPA Submission:true Bankit Comment: TPA EVIDENCE:The viral sequences were derived from a publicly available transcriptome of the host fungus. This transcriptome is available on NCBI Sequencing Reads Archive (SRA). Contigs were assembled from this dataset by using the assembler rnaViralSPAdes, which is optimized for the assembly of RNA viruses. To identify putative mycoviruses, these contigs were converted to protein sequences, and were then aligned to a custom viral database consisting of viral proteins of various mycoviruses from various viral families. Once these contigs were identified, they were further analysed using BLASTp, to distinguish actual mycoviruses from any fungal derived proteins. This particular virus, Thielaviopsis paradoxa RNA virus 1, matched closely to members of the viral family Mitoviridae, and contained an ORF which encodes for an RdRp domain. Bankit Comment: ALT EMAIL:bientjie.hough@gmail.com Bankit Comment: TOTAL # OF SEQS:1 ##Assembly-Data-START## Assembly Method :: rnaViralSPAdes v. 3.15.1 Assembly Name :: Thielaviopsis_paradoxa_RNA_Virus_1 Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..2599 /organism="Thielaviopsis paradoxa RNA Virus 1" /mol_type="genomic RNA" /strain="T-189" /host="Thielaviopsis paradoxa T-189" /country="South Africa" /note="Viruses; Riboviria; Orthornavirae; Lenarviricota;Howeltoviricetes; Cryppavirales; Mitoviridae; Unuamitovirus" CDS 329..2575 /codon_start=1 /transl_table=4 /product="RNA dependent RNA polymerase" /translation="MKYTPQLIIERLINLIFNINGSMYVSRYLLLFNNMRKNNGLLYT IKWFKAAKLHITRYICGKPLLSNTEGVSVDKEGFPTRLDFLKPLVKENPRALLTLLSF TRSVEPTKEEEKYRKPKTDSIVAPYKGKEYTIPKSFIESWIKQFDLNLEKHSYSIKDH YLSMKGSPNGKSSISSLWSLAFHSMDTLGYIKYLCGDFYNQLSKVYINLWVHHRDLLE KKATKNTIGKLSVVHDPELKERVIAMVDYTTQFILRPIHDDLLSLLKKLPCDRTFTQS PFHDWLDSNDCYYSLDLSSATDRFPIQLQQKLIALIYKDYEFARNWKDLLVDRSYWFE SKEYRYSVGQPMGAYTSWAAFTLTHHLVVAWAAKLAGYSTFHDYIILGDDIVIHNDRV AQRYINLMNKFGVEISVPKTHVSRNTYEFAKRWIQNGKEITGLSLRGILTNIKHIHVV YMNIFTYLQRIPSLNVNVLKIVCQLYTNIKIGNRIKTSNSIFRSLYDFHHSIRYSFGL LTYEELRNYLINKIPFEEFIPWPMGSAHSRVNEILSEGIVDQAKALNSKLKSEFFKLE DFDLSSELMKTWPLTYGYHNHIQKLKSSLLSLVNGSTDLIDIISEVRLQNIDSILKFK RNTFENLILLDKLWKKAFKVYKDKLSKELVPKEENPYVPAGGLVKHIWGIPKSSPFIP YKSTHLGLKTWKEELETNINSLDRDLDFFFNAEVQPEHKEHIQDRIIAYVIDCKRKGI LPKFDDFK" BASE COUNT 945 a 410 c 406 g 838 t ORIGIN 1 taaaagggct agacgaaaca agccatggtt aagttttaaa ttaaccatcc agaatctttg 61 ttcacaaatg aagattcaaa gaatagaaac cagcaatggc cttctctatt atatgtggag 121 tagctttcta gtaatagatc gccaagtcca catgtataca tgttacaaga attaagtagg 181 gttgatcacc ctttgataaa attcttgtcc catataggac agtagtggca tcagatgtcc 241 gtccatatga agatatagat gaactacatt tagagttaaa agctcctgat gtagagaata 301 tatgatctta taattaataa caatcaacat gaaatacaca ccacaactaa taattgagag 361 actaataaac ttaatcttca atattaatgg tagtatgtat gtttcacgtt atttattgct 421 ctttaataat atgcgaaaga ataatggatt actttataca atcaaatgat ttaaagctgc 481 aaagcttcac atcacaagat atatatgcgg taaaccacta ttaagtaaca cagaaggagt 541 ttctgttgat aaagaggggt ttcctacgcg gttagatttt ctaaaaccat tggttaagga 601 aaatccaaga gcgcttctaa ctttattaag ttttacacgt agtgtagaac caactaaaga 661 agaagagaaa tatagaaaac caaagactga ttctattgta gcaccctata aaggaaaaga 721 gtatactata cccaaatcat ttatagaatc ctgaataaaa cagtttgatc taaatcttga 781 gaaacattca tatagtatta aagatcatta cctaagtatg aaaggttctc ctaacggaaa 841 atcttctata tcaagtttat gatctttagc tttccactca atggacacac ttggatatat 901 aaaatatctt tgtggtgatt tctataacca actttcgaag gtttatataa atctttgagt 961 tcaccataga gatcttcttg aaaagaaggc cactaagaat actattggaa aactatctgt 1021 ggtacatgac cctgagttaa aagaaagagt aattgcaatg gtagactata ccactcaatt 1081 tattcttcgt cctatacatg atgatttatt aagtttactt aagaaattac cttgtgatag 1141 aacttttact caaagtcctt tccatgattg attagacagc aatgattgct actattcact 1201 tgacctttct tctgcaactg atagatttcc tatacaatta caacaaaaat tgatagcctt 1261 gatttataaa gactatgaat ttgcgcgtaa ttgaaaggat ttacttgtag atcgttccta 1321 ttgatttgaa tcaaaagaat atcgctattc tgttggtcaa ccaatggggg cttatactag 1381 ttgagctgct ttcacattaa cacatcacct tgttgtagct tgagctgcca aattggcagg 1441 ttattctaca tttcatgatt atataatcct aggtgacgat atcgtaattc acaacgatag 1501 agtcgcacaa agatatataa acttgatgaa caagtttggt gttgaaatca gtgttccaaa 1561 aactcatgta agtagaaata cttatgagtt tgcgaaacgt tgaattcaaa atggaaaaga 1621 aatcactggt ctatctttaa gaggaattct tacaaatata aaacatatcc atgtagtcta 1681 catgaatata tttacttatt tacaaagaat tccatcctta aatgtgaacg tcttaaagat 1741 tgtatgtcaa ttatatacta atataaagat tggaaataga attaaaactt ctaattccat 1801 ctttagatca ctatatgatt tccatcattc tataagatac tcatttggtc ttcttactta 1861 cgaggagttg cgaaactact tgataaataa gataccattt gaggagttta ttccatgacc 1921 tatgggatca gctcattcac gagtgaatga aatcttgtca gagggaatag ttgatcaagc 1981 taaagcttta aattcaaaat taaaatcaga attcttcaaa cttgaagatt ttgatcttag 2041 ttctgaactt atgaagacct gacctttaac atatggttat cataaccaca tacaaaagtt 2101 aaaatcttca ctactttcat tagtaaacgg atcaacagat ttaatagata ttatttctga 2161 agttcgttta caaaatatag atagtattct gaaattcaaa cgtaatacat ttgaaaatct 2221 gatacttcta gataaactat gaaagaaagc ttttaaagta tataaggata aactttcaaa 2281 ggaactagtt cctaaagaag aaaatcctta tgtacctgcg ggtggattag taaaacatat 2341 ctggggaatt cctaagagtt caccatttat accttataaa tctactcacc ttggtctgaa 2401 gacatgaaaa gaagaattag agaccaatat caattcactt gatagagatc ttgacttctt 2461 ttttaatgcc gaagttcaac ctgagcataa agaacatata caagatcgta ttatagccta 2521 tgttatagac tgtaaacgaa aaggtatact acctaaattt gatgatttta agtaattatc 2581 tctggagtta aactccctt //