Chemical blood studies VIII. A rapid spectroscopic method for (A) the quantitative determination of haemoglobin in blood and (B) its application for the quantitative estimation of haemoglobin in milk, urine, serum or plasma and faeces

Abstract

A spectroscopic method for the quantitative determination of the haemoglobin content of blood is described. The haematin is converted into pyridine haemochromogen and a comparison of the intensity of the 555 absorption band made against a known standard. Haemoglobin can be quantitatively estimated in milk, urine, serum, plasma or faeces.