Abstract:
Monozygotic twinning has not previously been genetically confirmed in the dog. This case report
describes the finding of two viable male monozygotic foetuses within one placental site during
caesarean section. Their umbilical cords attached to a single placenta. Genetic profiling using a
total of 38 microsatellite markers, as well as amelogenin and SRY for sex determination, revealed
identical DNA profiles, whether derived from blood or tissue (buccal swabs) samples. To the best
of our knowledge, this is the first report of monozygotic twinning in the dog confirmed using DNA
profiling.
