dc.contributor.author |
Dzobo, Kevin
|
|
dc.contributor.author |
Vogelsang, Matjaz
|
|
dc.contributor.author |
Thomford, Nicholas Ekow
|
|
dc.contributor.author |
Dandara, Collet
|
|
dc.contributor.author |
Kallmeyer, Karlien
|
|
dc.contributor.author |
Pepper, Michael Sean
|
|
dc.contributor.author |
Parker, M. Iqbal
|
|
dc.date.accessioned |
2016-08-18T06:58:35Z |
|
dc.date.available |
2016-08-18T06:58:35Z |
|
dc.date.issued |
2016 |
|
dc.description.abstract |
The tumour microenvironment plays a crucial role in tumour progression and comprises tumour stroma which is made up of
different cell types and the extracellular matrix (ECM).Mesenchymal stromal cells (MSCs) are part of the tumour stroma and may
have conflicting effects on tumour growth. In this study we investigated the effect of Wharton’s Jelly-derived MSCs (WJ-MSCs)
and a fibroblast-derived ECM (fd-ECM) on esophageal (WHCO1) and breast (MDAMB 231) cancer cells in vitro. BothWJ-MSCs
and the fd-ECM, alone or in combination, downregulate PCNA, cyclin D1, Bcl-2, Bcl-xL, and MMPs and upregulate p53 and p21.
p21 induction resulted in G2 phase cell cycle arrest and induced apoptosis in vitro. Our data suggest that p21 induction is via p53-
dependent and p53-independent mechanisms inWHCO1 andMDA MB 231 cells, respectively. Vascular endothelial growth factor,
Akt, and Nodal pathways were downregulated in cancer cells cocultured with WJ-MSCs. We also demonstrate that WJ-MSCs
effects on cancer cells appear to be short-lived whilst the fd-ECM effect is long-lived. This study shows the influence of tumour
microenvironment on cancer cell behaviour and provides alternative therapeutic targets for potential regulation of tumour cells. |
en_ZA |
dc.description.department |
Immunology |
en_ZA |
dc.description.librarian |
am2016 |
en_ZA |
dc.description.sponsorship |
The International Centre for Genetic Engineering and Biotechnology (ICGEB), the South
African Medical Research Council, the National Research
Foundation (NRF) of South Africa, theUniversity of Pretoria,
and the University of Cape Town. Karlien Kallmeyer and
Michael S. Pepper’s work was funded by the South African
Medical Research Council (University Flagship award and
Extramural Stem Cell Unit). |
en_ZA |
dc.description.uri |
http://www.hindawi.com/journals/sci/ |
en_ZA |
dc.identifier.citation |
Dzobo, K, Vogelsang, M, Thomford, NE, Dandara, C, Kallmeyer, K, Pepper, MS & Parker, MI 2016, 'Wharton’s jelly-derived mesenchymal stromal cells and fibroblast-derived extracellular matrix synergistically activate apoptosis in a p21-dependent mechanism in WHCO1 and MDA MB 231 cancer cells in vitro', Stem Cells International, vol. 2016, pp. 1-17. |
en_ZA |
dc.identifier.issn |
1687-966X (print) |
|
dc.identifier.issn |
1687-9678 (online) |
|
dc.identifier.other |
10.1155/2016/4842134 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/56389 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Hindawi Publishing Corporation |
en_ZA |
dc.rights |
© 2016 Kevin Dzobo et al.This is an open access article distributed under the Creative Commons Attribution License. |
en_ZA |
dc.subject |
Tumour microenvironment |
en_ZA |
dc.subject |
In vitro |
en_ZA |
dc.subject |
Tumour cells |
en_ZA |
dc.subject |
Extracellular matrix (ECM) |
en_ZA |
dc.subject |
Mesenchymal stromal/stem cells (MSCs) |
en_ZA |
dc.subject |
Wharton’s Jelly-derived MSCs (WJ-MSCs) |
en_ZA |
dc.subject |
Fibroblast-derived ECM (fd-ECM) |
en_ZA |
dc.subject |
Esophageal (WHCO1) |
en_ZA |
dc.subject |
Breast (MDAMB 231) |
en_ZA |
dc.subject |
Cancer cells |
en_ZA |
dc.title |
Wharton’s jelly-derived mesenchymal stromal cells and fibroblast-derived extracellular matrix synergistically activate apoptosis in a p21-dependent mechanism in WHCO1 and MDA MB 231 cancer cells in vitro |
en_ZA |
dc.type |
Article |
en_ZA |