The partial purification of Clostridium perfringens Beta toxin

Mulders, M.S.G.; Worthington, R.W.

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dc.contributor.author	Mulders, M.S.G.
dc.contributor.author	Worthington, R.W.
dc.contributor.editor	Bigalke, R.D.
dc.contributor.editor	Cameron, Colin McKenzie
dc.contributor.editor	Verster, Anna J,M.
dc.contributor.editor	Walker, Jane B.
dc.contributor.other	De Kock, V.E.
dc.date.accessioned	2016-07-06T11:10:13Z
dc.date.available	2016-07-06T11:10:13Z
dc.date.created	2016
dc.date.issued	1975
dc.description	The articles have been scanned in colour with a HP Scanjet 5590; 300dpi. Adobe Acroabt XI was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.	en_ZA
dc.description.abstract	An attempt was made to purify Clostridium perfringens Beta toxin. Crude toxin prepared by ammonium sulphate precipitation of culture supernatants was purified by chromatography on Sephadex G50, Sephadex G100 and DEAE cellulose. This material, although highly purified, was not homogeneous on polyacrylamide gel electrophoresis. It had a toxicity of 800 000 mouse MLDs/mg N, a typical protein absorption spectrum in the UV region, an iso-electric point of 5, 6 and the main component had a molecular mass of 42 000 ± 2 000 (estimated by electrophoresis in sodium dodecyl sulphate containing polyacrylamide gels)	en_ZA
dc.identifier.citation	Worthington, RW & Mulders, MSG 1975, 'The partial purification of Clostridium perfringens Beta toxin’, Onderstepoort Journal of Veterinary Research, vol. 42, no. 3, pp. 91-98.	en_ZA
dc.identifier.issn	0330-2465
dc.identifier.uri	http://hdl.handle.net/2263/53723
dc.language.iso	en	en_ZA
dc.publisher	Published by The Government Printer, Pretoria	en_ZA
dc.rights	©1975 ARC-Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©2016 University of Pretoria. Department of Library Services (digital).	en_ZA
dc.subject	Veterinary medicine	en_ZA
dc.subject.lcsh	Veterinary medicine -- South Africa
dc.title	The partial purification of Clostridium perfringens Beta toxin	en_ZA
dc.type	Article	en_ZA