The study aimed to determine the presence of mumps virus (MuV) in cerebrospinal fluid (CSF) specimens and to genetically
characterise detected MuV strains. A real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the
MuV F gene, and characterisation was performed by sequencing of the SH gene. Mumps virus was detected in 1.2% (3/260) of
specimens. Phylogenetic analysis of one MuV strain revealed that it clustered with the Jeryl-Lynn and RIT4385 vaccine strains.
As far as the authors could ascertain this is the first study to provide viral proof that these vaccine-like strains may be associated
with aseptic meningitis.
The information contained in this article was
presented by Marieke Brauer at the UNIPATH 2014 conference, held on 19-21 September 2014 at the CSIR Convention Centre in
Pretoria (no abstract number available).