Abstract:
We have undertaken an in-depth transcriptome analysis of adipogenesis in human adipose-derived stromal cells
(ASCs) induced to differentiate into adipocytes in vitro. Gene expression was assessed on days 1, 7, 14 and 21
post-induction and genes differentially expressed numbered 128, 218, 253 and 240 respectively. Up-regulated
genes were associated with blood vessel development, leukocyte migration, as well as tumor growth, invasion
and metastasis. They also shared common pathways with certain obesity-related pathophysiological conditions.
Down-regulated genes were enriched for immune response processes. KLF15, LMO3, FOXO1 and ZBTB16 transcription
factors were up-regulated throughout the differentiation process. CEBPA, PPARG, ZNF117, MLXIPL,
MMP3 and RORB were up-regulated only on days 14 and 21, which coincide with the maturation of adipocytes
and could possibly serve as candidates for controlling fat accumulation and the size of mature adipocytes. In summary,
we have identified genes that were up-regulated only on days 1 and 7 or days 14 and 21 that could serve as
potential early and late-stage differentiation markers.
