Culicoides spp. in the Lowveld of the northern Transvaal, Republic of South Africa, were fed
bluetongue virus serotypes 3 and 6 and African horsesickness virus serotype 1 through latex and chicken
skin membranes. After an incubation period of 10 days at 25-27 °C, the infection rate of C. imicola for
bluetongue virus serotypes 3 and 6 was established at 31% and 24% respectively. No African horsesickness
virus could be recovered. The membrane feeding technique and handling procedures proved to be suitable for field studies
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