dc.contributor.author |
Nieuwoudt, Martin J.
|
|
dc.contributor.author |
Lameris, Roeland
|
|
dc.contributor.author |
Corcoran, Craig
|
|
dc.contributor.author |
Rossouw, Theresa M.
|
|
dc.contributor.author |
Slavik, Tomas
|
|
dc.contributor.author |
Du Plessis, Johannie
|
|
dc.contributor.author |
Omoshoro-Jones, Jones A.O.
|
|
dc.contributor.author |
Stivaktas, Paraskevi Irene
|
|
dc.contributor.author |
Potgieter, Fritz
|
|
dc.contributor.author |
Van der Merwe, Schalk Willem
|
|
dc.date.accessioned |
2014-08-13T06:43:49Z |
|
dc.date.available |
2014-08-13T06:43:49Z |
|
dc.date.issued |
2014-09 |
|
dc.description.abstract |
Abdominal lymphadopathy in Human Immunodeficiency Virus (HIV) infection
remains a diagnostic challenge. We performed a prospective cohort study
recruiting thirty-one symptomatic HIV+ patients with abdominal
lymphadenopathy assessing diagnostic yield of endoscopic ultrasound (EUS)
fine needle aspiration (FNA). Mean age was 38 years, 52% were female, mean
CD4 count and viral load were 124 cells/pl, and 4 log respectively. EUS
confirmed additional mediastinal nodes in 26 %. Porta- hepatis was the most
common abdominal site. EUS FNA was subjected to cytology, culture and
polymerase chain reaction (PCR) analysis. Mycobacterial infections were
confirmed in 67.7% and 31% had reactive lymphadenopathy. Cytology and
culture had low sensitivity whereas PCR identified 90% of mycobacterial
infections. Combining appearance of EUS FNA and cytology a diagnostic
algorithm was developed to indicate when analysis with PCR would be useful.
PCR performed on an EUS guided aspirate was highly accurate in confirming
mycobacterial disease and determining genotypic drug resistance. |
en_US |
dc.description.librarian |
hb2014 |
en_US |
dc.description.sponsorship |
South African Gastroenterological Society (SAGES)/ Astra Zeneca Fellowship in Gastroenterology awarded to Schalk van der Merwe |
en_US |
dc.description.uri |
http://www.journals.elsevier.com/ultrasound-in-medicine-and-biology/ |
en_US |
dc.identifier.citation |
Nieuwoudt, M, Lameris, R, Corcoran, C, Rossouw, TM, Slavik, T, Du Plessis, J, Omoshoro-Jones, JAO, Stivaktas, PI, Potgieter, F & Van der Merwe, SW 2014, 'Polymerase chain reaction amplifying mycobacterial DNA from aspirates obtained by endoscopic ultrasound allows accurate diagnosis of mycobacterial disease in HIV-positive patients with abdominal lymphadenopathy', Ultrasound in Medicine & Biology, vol. 40, no. 9, pp. 2031-2038. |
en_US |
dc.identifier.issn |
0301-5629 (print) |
|
dc.identifier.issn |
1879-291X (online) |
|
dc.identifier.other |
10.1016/j.ultrasmedbio.2014.04.006 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/41226 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
Elsevier |
en_US |
dc.rights |
© 2014 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Ultrasound in Medicine and Biology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Ultrasound in Medicine and Biology, vol. 40, no. 9, pp. 2031-2038, 2014. doi : 10.1016/j.ultrasmedbio.2014.04.006. |
en_US |
dc.subject |
Abdominal lymphadenopathy |
en_US |
dc.subject |
Endoscopic ultrasound |
en_US |
dc.subject |
Human immunodeficiency virus (HIV) |
en_US |
dc.subject |
Tuberculosis (TB) |
en_US |
dc.title |
Polymerase chain reaction amplifying mycobacterial DNA from aspirates obtained by endoscopic ultrasound allows accurate diagnosis of mycobacterial disease in HIV-positive patients with abdominal lymphadenopathy |
en_US |
dc.type |
Postprint Article |
en_US |