dc.contributor.author |
Seoposengwe, Keabetswe
|
|
dc.contributor.author |
Van Tonder, Jacob John
|
|
dc.contributor.author |
Steenkamp, Vanessa
|
|
dc.date.accessioned |
2014-03-07T09:38:11Z |
|
dc.date.available |
2014-03-07T09:38:11Z |
|
dc.date.issued |
2013-12-12 |
|
dc.description.abstract |
BACKGROUND: Lannea schweinfurthii, Zanthoxylum capense, Scadoxus puniceus and Crinum bulbispermum are used
traditionally to treat neurological disorders. The aim of this study was to evaluate the cytoprotective potential of the
four plants, after induction of toxicity using rotenone, in SH-SY5Y neuroblastoma cells.
METHODS: Cytotoxicity of the plant extracts and rotenone was assessed using the sulforhodamine B (SRB) assay.
Fluorometry was used to measure intracellular redox state (reactive oxygen species (ROS) and intracellular glutathione
content), mitochondrial membrane potential (MMP) and caspase-3 activity, as a marker of apoptotic cell death.
RESULTS : Of the tested plants, the methanol extract of Z. capense was the least cytotoxic; LC50 121.3 ± 6.97 μg/ml, while
S. puniceus methanol extract was the most cytotoxic; LC50 20.75 ± 1.47 μg/ml. Rotenone reduced intracellular ROS levels
after 24 h exposure. Pre-treating cells with S. puniceus and C. bulbispermum extracts reversed the effects of rotenone on
intracellular ROS levels. Rotenone exposure also decreased intracellular glutathione levels, which was counteracted by
pre-treatment with any one of the extracts. MMP was reduced by rotenone, which was neutralized by pre-treatment
with C. bulbispermum ethyl acetate extract. All extracts inhibited rotenone-induced activation of caspase-3.
CONCLUSION : The studied plants demonstrated anti-apoptotic activity and restored intracellular glutathione content
following rotenone treatment, suggesting that they may possess neuroprotective properties. |
en_US |
dc.description.librarian |
am2014 |
en_US |
dc.description.sponsorship |
The National Research
Foundation (NRF) and the University of Pretoria, Research Committee,
Faculty of Health Sciences (RESCOM) |
en_US |
dc.description.uri |
http://www.biomedcentral.com/1472-6882/13/353 |
en_US |
dc.identifier.citation |
Seoposengwe et al.: In vitro neuroprotective potential of four medicinal plants against rotenone-induced toxicity in SH-SY5Y neuroblastoma cells. BMC Complementary and Alternative Medicine 2013 13:353. |
en_US |
dc.identifier.issn |
1472-6882 |
|
dc.identifier.issn |
10.1186/1472-6882-13-353 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/37090 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
BioMed Central |
en_US |
dc.rights |
© 2013 Seoposengwe et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the
Creative Commons Attribution License |
en_US |
dc.subject |
Caspase-3 |
en_US |
dc.subject |
Crinum bulbispermum |
en_US |
dc.subject |
Glutathione |
en_US |
dc.subject |
Lannea schweinfurthii |
en_US |
dc.subject |
Mitochondrial membrane potential |
en_US |
dc.subject |
Neuroprotection |
en_US |
dc.subject |
Reactive oxygen species (ROS) |
en_US |
dc.subject |
Rotenone |
en_US |
dc.subject |
Scadoxus puniceus |
en_US |
dc.subject |
Zanthoxylum capense |
en_US |
dc.title |
In vitro neuroprotective potential of four medicinal plants against rotenone-induced toxicity in SH-SY5Y neuroblastoma cells |
en_US |
dc.type |
Article |
en_US |