dc.contributor.author |
Pretorius, A.
|
|
dc.contributor.author |
Oelofsen, M.J.
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|
dc.contributor.author |
Smith, M.S.
|
|
dc.contributor.author |
Van der Ryst, E.
|
|
dc.contributor.editor |
Verwoerd, Daniel Wynand |
|
dc.date.accessioned |
2012-12-12T08:56:50Z |
|
dc.date.available |
2012-12-12T08:56:50Z |
|
dc.date.created |
2012 |
|
dc.date.issued |
1997 |
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dc.description |
The articles have been scanned in colour with a HP Scanjet 5590; 600dpi.
Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. |
en_US |
dc.description.abstract |
Infectious diseases of wild animals are of increasing importance, both from an economic viewpoint and because several of these diseases are pathogenic to man. However, serosurveys to determine the circulation of infectious organisms in wildlife are complicated by the fact that antibodies to species-specific immunoglobulins are not available for use in serological assays such as enzyme-linked immunosorbent assays (ELISAs) or immunofluorescence assays. To determine the binding potential of four commercially available antibody conjugates with the sera of wild animals, sera from 27
species of small terrestrial mammals were allowed to react with alkaline phosphatase-labelled protein A, anti-rabbit lgG, anti-mouse lgG and anti-human lgG by by the use of an ELISA. It was found that sera from some species of the order Lagomorpha bound optimally to anti-rabbit lgG, while anti-mouse lgG could be used for most species of Rodentia. For all Carnivora, lnsectivora, Macroscelidea, Hyracoidea and other Rodentia, staphylococcal protein A demonstrated optimal binding. None of the sera that was tested bound to anti-human lgG. These results demonstrate that commercial conjugates can be used in serological assays in which wild animal sera are used, and should be useful
for future serosurveys to determine the circulation of infectious agents in small terrestrial mammals. |
en_US |
dc.identifier.citation |
Pretorius, A, Oelofsen, MJ, Smith, MS & Van der Ryst, E 1997, 'The binding potential of commercial antibody conjugates with sera of various small terrestrial mammals’. Onderstepoort Journal of Veterinary Research, vol. 64, no. 3, pp. 201-203. |
en_US |
dc.identifier.issn |
0330-2465 |
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dc.identifier.uri |
http://hdl.handle.net/2263/20749 |
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dc.language.iso |
en |
en_US |
dc.publisher |
Published by the Agricultural Research Council, Onderstepoort Veterinary Institute |
en_US |
dc.rights |
© ARC-Onderstepoort (original).
© University of Pretoria. Dept of Library Services (digital). |
en_US |
dc.subject |
Veterinary medicine |
en_US |
dc.subject |
Antibody conjugates |
en_US |
dc.subject |
Binding potential |
en_US |
dc.subject |
Commercial cojugates |
en_US |
dc.subject |
ELISA |
en_US |
dc.subject |
Serosurvey |
en_US |
dc.subject.lcsh |
Veterinary medicine -- South Africa |
|
dc.title |
The binding potential of commercial antibody conjugates with sera of various small terrestrial mammals |
en_US |
dc.type |
Article |
en_US |