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2002 Onderstepoort Journal of Veterinary Research, Volume 69, 2002: Recent submissions

  • Development of a diagnostic one-tube RT-PCR for the detection of Rift Valley fever virus 
    Nel, Louis Hendrik; Espach, Anel; Romito, M.; Viljoen, G.J.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Diagnosis of Rift Valley fever (RVF) is based on serology and virus isolation. The disadvantages of the former include poor sensitivity, high cost, risks associated with using infectious virus as antigen, the lengthy ...
  • The effect of three bufadienolide cardiac glycosides on contraction of isolated rat jejunum 
    Botha, C.J. (Christoffel Jacobus); Gehring, Ronette; Van Rooyen, J.M.; Venter, D.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Three cardiac glycosides were screened for pharmacological effects on isolated rat jejunum. The contraction of rat jejunum with epoxyscillirosidin, a non-cumulative bufadienolide, and cotyledoside and tyledoside D, both ...
  • Water absorption in relation to fermentation in the colon of the ostrich (Struthio camelus) 
    Musara, C.; Chamunorwa, Joseph Panashe; Holtug, K.; Skadhauge, E.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    The colon is a major site for fermentation and water absorption in the ostrich. Water absorption along the colon was evaluated and its relationship to osmolality, Na+ concentration, short chain fatty acid (SCFA) concentration ...
  • The Kumm isolate of Ehrlichia ruminantium: in vitro isolation, propagation and characterization 
    Zweygarth, Erich; Van Heerden, Henriette; Allsopp, Basil A.; Josemans, A.I.; Van Strijp, M.F.; Allsopp, M.T.E.P.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    An effective culture system for Ehrlichia (Cowdria) ruminantium comb. nov. was first established in 1985 and many stocks were subsequently isolated and propagated in vitro. A notable exception, however, was the Kumm isolate ...
  • Non-immune control of trypanosomosis: in vitro oxidative burst of PMA- and trypanosome-stimulated neutrophils of Boran and N'Dama cattle 
    Taiwo, V.O.; Adejinmi, J.O.; Oluwaniyi, J.O.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    An in vitro assay that measures the generation of superoxide anions (O₂ˉ) was used to assess the level of oxidative burst of phorbol myristate acetate (PMA) - and trypanosome-stimulated neutrophils isolated from healthy ...
  • Subject index 
    Anonymous; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
  • Author index 
    Anonymous; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
  • Review of current problems and shortcomings in the Tanzanian animal health information system with suggestions on improvement 
    Kivaria, F.M.; Kapaga, A.M.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Livestock diseases have always been the focus of the Tanzanian Veterinary Authorities. However, they have become more important since the formation of the World Trade Organisation and subsequent implementation of the various ...
  • Description of Pelonia africana n. g., n. sp. (Pentastomida: Sebekidae) from the lungs of Pelomedusa subrufa and Pelusios sinuatus (Chelonia) in South Africa 
    Boomker, Jacob Diederik Frederik; Junker, Kerstin; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    The terrapins Pelomedusa subrufa and Pelusios sinuatus taken from a water reservoir in the Northern Province, South Africa, were examined for pentastome infections. Two immature specimens, a patent female and a mature male, ...
  • Teratogenicity of a mutagenised Rift Valley fever virus (MVP 12) in sheep 
    Hunter, P.; Erasmus, B.J.; Vorster, J.H. (Jan Harm); Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    A 5-fluorouracil mutagenised Rift Valley fever virus strain, which was shown to be attenuated and immunogenic in cattle and sheep, was evaluated for its ability to cause teratogenic effects in pregnant sheep. A group of ...
  • Amphistome infection of goats farmed under resource-poor conditions in South Africa 
    Vatta, A.F.; Krecek, R.C.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    From December 1998 to April 2000, a longitudinal study was conducted of the pooled trematode faecal egg counts of samples collected from goats of resource-poor farmers at Rust de Winter, Gauteng Province, Impendle, ...
  • Continuous exposure to an aversive mixture as a means of maintaining aversion to vermeerbos (Geigeria ornativa O. Hoffm.) in the presence of non-averted sheep 
    Snyman, Leendert D.; Schultz, R.A. (Rowena Anitra); Kellerman, T. Stephanus; Labuschagne, Leonie; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Continuous exposure to an aversive mixture was investigated as a means of maintaining aversion to vermeerbos in sheep subjected to the social influence of non-averted sheep. The use of an aversive mixture was based on a ...
  • Multiplication of the V4 strain of Newcastle disease virus in Madin Derby bovine kidney cells 
    Sahle, M.; Burgess, W.G.; Kidanemariam, Awoke; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    This study describes a reproducible cell culture system that permits the growth and secondary multiplication of the V4 strain of Newcastle disease virus. Allantoic fluid, magnesium chloride and diethylaminoethyl dextran ...
  • The classification of seven serotypes of equine encephalosis virus and the prevalence of homologous antibody in horses in South Africa 
    Howell, P.G.; Bosman, Anna-Mari; Coetzer, Jacobus A.W.; Guthrie, Alan John; Groenewald, Daleen; Visage, Carina W.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Selected isolates of equine encephalosis virus were shown to have comparable viral protein profiles and to represent seven distinct serotypes, based on cross-neutralization tests. Serotype-specific virus-neutralizing ...
  • Apparent prevalence of dourine in the Khomas region of Namibia 
    Kumba, F.F.; Claasen, B.; Petrus, P.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    A 15-year record of the results of horse sera from the Khomas region of Namibia tested by the complement fixation test for dourine at the Central Veterinary Laboratory in Windhoek before clearing the respective animals for ...
  • Book review 
    Anonymous; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
  • Book review 
    Anonymous; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
  • The prevalence and economic importance of bovine fasciolosis in Kenya - an analysis of abattoir data 
    Kithuka, J.M.; Maingi, N.; Njeruh, F.M.; Ombui, J.N.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    A retrospective study covering a period of 10 years (1990-1999) was carried out using post mortem meat inspection records at the Veterinary Department Headquarters at Kabete to determine the prevalence and economic importance ...
  • An overview of the eradication of Brucella melitensis from KwaZulu-Natal 
    Emslie, Forbes Richard; Nel, J.R.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Brucella melitensis is a Gram-negative bacterium whose primary hosts are goats and sheep. Like the other Brucella spp., with the exception of Brucella ovis, it is not particularly host specific as it is pathogenic for a ...
  • Effects of the scab mite Psoroptes ovis on the haematology and live mass of Merino and Dorper sheep 
    Horak, Ivan Gerard; Meintjes, T.; Fourie, L.J.; Boomker, Jacob Diederik Frederik (Pretoria : Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Five Merino and five Dorper sheep were artificially infested with the sheep scab mite Psoroptes ovis and the effect of infestation on their haematology, serum protein levels and live mass recorded for a period of 14 weeks. ...