Abstract:
Comprehensive Gas Chromatography–Time-of-Flight Mass Spectrometry (GC×GC–TOFMS) methodology
has been refined for the analysis of polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated
dibenzofurans (PCDFs) in samples with different matrices. This is specifically for application in developing
countries where access to gas chromatography–high resolution mass spectrometry (GC–HRMS)
and highly skilled personnel is limited. The method, using an Rxi-5 Sil MS column in the first dimension
(D) coupled with an Rtx-200 column in the second dimension (D), was used to quantify PCDDs
and PCDFs in different environmental sample matrices. The results were compared with those obtained
usingGC–HRMSand good agreement was observed. The limit of detection (LOD) for the method (300 fg on
column for spiked soil samples) was determined using an Rxi-XLB (1D) column coupled with an Rtx-200
column (D). Preliminary South African sample results are also discussed. Isomer specificity for different
tetrachloro dibenzo-p-dioxins (TCDDs) and tetrachloro dibenzofurans (TCDFs) was investigated using a
commercial standard. Adequate resolution was achieved. The method as described has great attraction
for developing countries being both financially and operationally favourable.
